使用氧化石墨烯基质改善人雪旺样脂肪来源干细胞的胶质细胞分化。
Improving the glial differentiation of human Schwann-like adipose-derived stem cells with graphene oxide substrates.
作者信息
Verre Andrea Francesco, Faroni Alessandro, Iliut Maria, Silva Claudio, Muryn Cristopher, Reid Adam J, Vijayaraghavan Aravind
机构信息
School of Materials and National Graphene Institute, University of Manchester, Manchester M13 9PL, UK.
Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PL, UK.
出版信息
Interface Focus. 2018 Jun 6;8(3):20180002. doi: 10.1098/rsfs.2018.0002. Epub 2018 Apr 20.
Abstract
There is urgent need to improve the clinical outcome of peripheral nerve injury. Many efforts are directed towards the fabrication of bioengineered conduits, which could deliver stem cells to the site of injury to promote and guide peripheral nerve regeneration. The aim of this study is to assess whether graphene and related nanomaterials can be useful in the fabrication of such conduits. A comparison is made between graphene oxide (GO) and reduced GO substrates. Our results show that the graphene substrates are highly biocompatible, and the reduced GO substrates are more effective in increasing the gene expression of the biomolecules involved in the regeneration process compared to the other substrates studied.
摘要
迫切需要改善周围神经损伤的临床治疗效果。许多努力都致力于生物工程导管的制造，这种导管可以将干细胞输送到损伤部位，以促进和引导周围神经再生。本研究的目的是评估石墨烯及相关纳米材料是否可用于制造此类导管。对氧化石墨烯（GO）和还原氧化石墨烯基底进行了比较。我们的结果表明，石墨烯基底具有高度的生物相容性，与其他研究的基底相比，还原氧化石墨烯基底在增加参与再生过程的生物分子的基因表达方面更有效。
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本文引用的文献
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Sci Rep. 2016 May 20;6:25917. doi: 10.1038/srep25917.
2
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Eur J Neurosci. 2016 Feb;43(3):417-30. doi: 10.1111/ejn.13055. Epub 2015 Sep 18.
3
Engineered neural tissue with aligned, differentiated adipose-derived stem cells promotes peripheral nerve regeneration across a critical sized defect in rat sciatic nerve.具有定向分化脂肪来源干细胞的工程化神经组织促进大鼠坐骨神经临界尺寸缺损处的周围神经再生。
Biomaterials. 2015 Jan;37:242-51. doi: 10.1016/j.biomaterials.2014.10.009. Epub 2014 Oct 23.
4
Cell reprogramming into the pluripotent state using graphene based substrates.使用基于石墨烯的基底将细胞重编程为多能状态。
Biomaterials. 2014 Sep;35(29):8321-9. doi: 10.1016/j.biomaterials.2014.05.096. Epub 2014 Jul 2.
5
Collagen (NeuraGen®) nerve conduits and stem cells for peripheral nerve gap repair.胶原（NeuraGen®）神经导管和干细胞用于周围神经间隙修复。
Neurosci Lett. 2014 Jun 20;572:26-31. doi: 10.1016/j.neulet.2014.04.029. Epub 2014 May 2.
6
Biomimetic choline-like graphene oxide composites for neurite sprouting and outgrowth.仿生胆碱型氧化石墨烯复合材料促进神经突发芽和生长。
ACS Appl Mater Interfaces. 2013 Dec 26;5(24):13188-97. doi: 10.1021/am4042004. Epub 2013 Dec 6.
7
Effects of surface charges of graphene oxide on neuronal outgrowth and branching.氧化石墨烯表面电荷对神经元生长和分支的影响。
Analyst. 2014 Jan 7;139(1):105-15. doi: 10.1039/c3an01796f. Epub 2013 Oct 25.
8
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J Biomed Mater Res A. 2014 Oct;102(10):3419-28. doi: 10.1002/jbma.35001. Epub 2013 Oct 29.
9
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