腹腔注射林德勒提取物对用阿霉素处理的NMRI小鼠卵母细胞发育的影响。
The effect of intra-peritoneal administration of Lindl. extract on development of NMRI mice oocytes treated with Doxorubicin.
作者信息
Jafarian Zahra, Eimani Hussein, Azarnia Mahnaz, Shahverdi Abdol-Hossein, Eftekhari-Yazdi Poopak, Kamalinejad Mohammad
机构信息
Department of Cell and Molecular Biology, Faculty of Biological Sciences Kharazmi University Tehran Iran.
Department of Embryology, Reproductive Biomedicine Center Royan Institute for Reproductive Biomedicine, ACECR Tehran Iran.
出版信息
Reprod Med Biol. 2012 Oct 7;12(2):57-63. doi: 10.1007/s12522-012-0138-5. eCollection 2013 Apr.
PURPOSE
The effect of water-alcohol Lindl. extract on development of mice oocytes treated with Doxorubicin (dox) was examined in this study.
METHODS
The mice were classified into four groups. Control group, mice injected intraperitoneally (IP) with saline. Extract group alone, mice treated with 200 mg/kg of body weight (bw), IP, twelve consecutive days. Dox group alone, mice were given dox, IP, 10 mg/kg bw. Experimental group treated with extract and dox together. Effect of the extract on the development of mice oocytes treated with dox were evaluated through assisted reproductive technology techniques (ARTs).
RESULTS
Developmental rate and blastocyst formation was improved by using the extract. A significant increase in in vitro developmental competence in comparison with dox group ( < 0.05) was observed.
CONCLUSIONS
The results of this study indicated that Lindl. extract could prevent dox toxicity of dox affecting both follicle or oocytes, and therefore it can result in improved embryo development which was observed in mice treated with dox plus Lindl. compared to mice treated with dox alone.
目的
本研究考察了水醇提取物对经阿霉素（多柔比星）处理的小鼠卵母细胞发育的影响。
方法
将小鼠分为四组。对照组，小鼠腹腔注射生理盐水。单独提取物组，小鼠按200毫克/千克体重腹腔注射提取物，连续12天。单独多柔比星组，小鼠腹腔注射多柔比星，剂量为10毫克/千克体重。实验组同时给予提取物和多柔比星。通过辅助生殖技术（ARTs）评估提取物对经多柔比星处理的小鼠卵母细胞发育的影响。
结果
使用提取物可提高发育率和囊胚形成率。与多柔比星组相比，体外发育能力显著提高（P<0.05）。
结论
本研究结果表明，提取物可预防多柔比星对卵泡或卵母细胞的毒性作用，因此与单独使用多柔比星处理的小鼠相比，在同时使用提取物和多柔比星处理的小鼠中观察到胚胎发育得到改善。
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