脂肪酸对公猪精子活力、生存力及顶体反应的影响。
Effect of fatty acids on boar sperm motility, viability and acrosome reaction.
作者信息
Hossain Md Sharoare, Tareq K M A, Hammano Ko-Ichi, Tsujii Hirotada
机构信息
Laboratory of Animal Biotechnology, Faculty of Agriculture, Shinshu University, Nagano, Japan.
出版信息
Reprod Med Biol. 2007 Nov 7;6(4):235-239. doi: 10.1111/j.1447-0578.2007.00191.x. eCollection 2007 Dec.
The present study was undertaken to determine which fatty acids improve motility, viability, and increase acrosome reaction (AR) in boar spermatozoa. Boar spermatozoa were washed, swum-up and incubated at 37°C for 4 h in TALP medium supplemented with myristic, palmitic, stearic, lignoceric, oleic, linoleic, arachidonic, docosahexaenoic and palmitoleic acid. Sperm motility, viability and AR were evaluated during 4 h of incubation. Results show that oleic and linoleic acid significantly improved ( 0.05) the motility and viability of boar spermatozoa. The AR was significantly improved ( 0.05) by oleic and arachidonic acid in almost all incubation periods. When combinations of oleic, linoleic and arachidonic acid were studied for motility, viability and AR, it was found that oleic plus linoleic acid significantly increased ( 0.05) motility, whereas arachidonic plus oleic acid significantly increased ( 0.05) AR. Unsaturated fatty acids, especially arachidonic acid, can improve boar sperm motility and AR. A combination of arachidonic and oleic acid is important for inducing boar sperm AR. (Reprod Med Biol 2007; 6: 235-239).
本研究旨在确定哪些脂肪酸可改善公猪精子的活力、存活率并增加顶体反应（AR）。公猪精子经洗涤、上浮处理后，在补充了肉豆蔻酸、棕榈酸、硬脂酸、二十四烷酸、油酸、亚油酸、花生四烯酸、二十二碳六烯酸和棕榈油酸的台氏液（TALP）培养基中于37℃孵育4小时。在孵育4小时期间评估精子活力、存活率和顶体反应。结果表明，油酸和亚油酸显著提高（P<0.05）了公猪精子的活力和存活率。在几乎所有孵育时间段，油酸和花生四烯酸均显著提高（P<0.05）了顶体反应。当研究油酸、亚油酸和花生四烯酸组合对活力、存活率和顶体反应的影响时，发现油酸加亚油酸显著提高（P<0.05）了活力，而花生四烯酸加油酸显著提高（P<0.05）了顶体反应。不饱和脂肪酸，尤其是花生四烯酸，可改善公猪精子活力和顶体反应。花生四烯酸和油酸组合对于诱导公猪精子顶体反应很重要。（《生殖医学与生物学》2007年；6：235 - 239）
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