基因-饮食相互作用在 CD36、NOS3 和 PPARG 对ω-3 脂肪酸补充剂对血脂的反应中的复制：一项双盲随机对照试验。
Replication of a Gene-Diet Interaction at CD36, NOS3 and PPARG in Response to Omega-3 Fatty Acid Supplements on Blood Lipids: A Double-Blind Randomized Controlled Trial.
机构信息
Institute of Basic Medical Sciences, Westlake Institute for Advanced Study, Westlake University, Hangzhou 310024, China; Institute of Nutrition and Health, Qingdao University, Qingdao 266071, China; Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China; MRC Epidemiology Unit, University of Cambridge, Cambridge CB20QQ, UK.
Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Center for Specialty Strategy Research of Shanghai Jiao Tong University China Hospital Development Institute, Shanghai 200011, China; Department of Clinical Nutrition, Zhejiang Hospital, Hangzhou 310000, China.
出版信息
EBioMedicine. 2018 May;31:150-156. doi: 10.1016/j.ebiom.2018.04.012. Epub 2018 Apr 17.
BACKGROUND
Modulation of genetic variants on the effect of omega-3 fatty acid supplements on blood lipids is still unclear.
METHODS
In a double-blind randomized controlled trial, 150 patients with type 2 diabetes (T2D) were randomized into omega-3 fatty acid group (n = 56 for fish oil and 44 for flaxseed oil) and control group (n = 50) for 180 days. All patients were genotyped for genetic variants at CD36 (rs1527483), NOS3 (rs1799983) and PPARG (rs1801282). Linear regression was used to examine the interaction between omega-3 fatty acid intervention and CD36, NOS3 or PPARG variants for blood lipids.
FINDINGS
Significant interaction with omega-3 fatty acid supplements was observed for CD36 on triglycerides (p-interaction = 0.042) and PPAGR on low-density lipoprotein-cholesterol (p-interaction = 0.02). We also found a significant interaction between change in erythrocyte phospholipid omega-3 fatty acid composition and NOS3 genotype on triglycerides (p-interaction = 0.042), total cholesterol (p-interaction = 0.013) and ratio of total cholesterol to high-density lipoprotein cholesterol (p-interaction = 0.015). The T2D patients of CD36-G allele, PPARG-G allele and NOS3-A allele tended to respond better to omega-3 fatty acids in improving lipid profiles. The interaction results of the omega-3 fatty acid group were mainly attributed to the fish oil supplements.
INTERPRETATION
This study suggests that T2D patients with different genotypes at CD36, NOS3 and PPARG respond differentially to intervention of omega-3 supplements in blood lipid profiles.
背景
ω-3 脂肪酸补充剂对血脂的影响的遗传变异的调节仍不清楚。
方法
在一项双盲随机对照试验中，将 150 名 2 型糖尿病（T2D）患者随机分为 ω-3 脂肪酸组（鱼油组 56 例，亚麻籽油组 44 例）和对照组（50 例），干预时间为 180 天。所有患者均对 CD36（rs1527483）、NOS3（rs1799983）和 PPARG（rs1801282）的遗传变异进行基因分型。线性回归用于检验 ω-3 脂肪酸干预与 CD36、NOS3 或 PPARG 变异对血脂的相互作用。
结果
ω-3 脂肪酸补充剂与 CD36 对甘油三酯（p 交互=0.042）和 PPARG 对低密度脂蛋白胆固醇（p 交互=0.02）的相互作用有显著意义。我们还发现，红细胞磷脂 ω-3 脂肪酸组成与 NOS3 基因型之间的变化与甘油三酯（p 交互=0.042）、总胆固醇（p 交互=0.013）和总胆固醇与高密度脂蛋白胆固醇的比值（p 交互=0.015）之间存在显著的相互作用。CD36-G 等位基因、PPARG-G 等位基因和 NOS3-A 等位基因的 T2D 患者对 ω-3 脂肪酸改善血脂谱的反应更好。ω-3 脂肪酸组的相互作用结果主要归因于鱼油补充剂。
结论
本研究提示，CD36、NOS3 和 PPARG 不同基因型的 T2D 患者对 ω-3 补充剂干预血脂谱的反应不同。
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