Lu Yan, Jiang Yun, Ling Lijun, Zhang Yunyi, Li Hong, Chen Daofeng
Department of Pharmacognosy, School of Pharmacy, Fudan University, Shanghai 201203, China.
Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China.
Acta Pharm Sin B. 2018 Mar;8(2):218-227. doi: 10.1016/j.apsb.2017.11.003. Epub 2017 Dec 8.
Thunb. is a traditional herb used for clearing heat and eliminating toxins, and has also been used for the treatment of severe acute respiratory syndrome (SARS). the crude polysaccharides (CHCP) exhibited potent anti-complementary activity through both the classical and alternative pathways by acting on components C3 and C4 of the complement system without interfering with the coagulation system. This study was to investigate the preventive effects of CHCP on acute lung injury (ALI) induced by hemorrhagic shock plus lipopolysaccharide (LPS) instillation (two-hit) and LPS-induced fever in rats. CHCP significantly attenuated pulmonary injury in the "two-hit" ALI model by reducing pulmonary edema and protein exudation in bronchoalveolar lavage fluid (BALF). In addition, it reduced the deposit of complement activation products in the lung and improved oxidant-antioxidant imbalance. Moreover, CHCP administration inhibited fever in rats, reduced the number of leukocytes and restored serum complement levels. The inhibition on the inappropriate activation of complement system by CHCP may play an important role in its beneficial effects on inflammatory diseases. The anti-complementary polysaccharides are likely to be among the key substances for the heat-clearing function of .
土牛膝是一种用于清热排毒的传统草药，也被用于治疗严重急性呼吸综合征（SARS）。粗多糖（CHCP）通过作用于补体系统的C3和C4成分，在经典途径和替代途径中均表现出强大的抗补体活性，且不干扰凝血系统。本研究旨在探讨CHCP对失血性休克加脂多糖（LPS）滴注（两次打击）诱导的急性肺损伤（ALI）以及LPS诱导的大鼠发热的预防作用。CHCP通过减少肺水肿和支气管肺泡灌洗液（BALF）中的蛋白质渗出，显著减轻了“两次打击”ALI模型中的肺损伤。此外，它减少了肺中补体激活产物的沉积，并改善了氧化-抗氧化失衡。此外，给予CHCP可抑制大鼠发热，减少白细胞数量并恢复血清补体水平。CHCP对补体系统不适当激活的抑制作用可能在其对炎症性疾病的有益作用中发挥重要作用。抗补体多糖可能是土牛膝清热功能的关键物质之一。
