Centro de Investigación y de Estudios Avanzados, Departamento de Biotecnología y Bioingeniería, Instituto Politécnico Nacional (Cinvestav-IPN), Unidad Zacatenco, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Ciudad de México 07360, Mexico.
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Mexico.
Molecules. 2022 May 6;27(9):2975. doi: 10.3390/molecules27092975.
Cedrela odorata L. is a plant species from the Meliaceae family that is cultivated for timber production. Although the C. odorata essential oil (EO) contains mainly sesquiterpenes, its insecticidal potential is unknown. The lipophilic properties and high degradation capacity of EOs have limited their application for use in pest control. However, the currently available knowledge on the nanoemulsification of EOs, in addition to the possibility of improving their dispersion, would allow them to prolong their permanence in the field. The objective of the present work was to develop a nanoemulsion of the C. odorata EO and to evaluate its larvicidal activity against Spodoptera frugiperda. The EO was obtained by the hydrodistillation of C. odorata dehydrated leaves, and the nanoemulsion was prepared with non-ionic surfactants (Tween 80 and Span 80) using a combined method of agitation and dispersion with ultrasound. The stability of the nanoemulsion with a droplet diameter of <200 nm was verified in samples stored at 5 °C and 25 °C for 90 days. Both the C. odorata EO and its corresponding nanoemulsion presented lethal properties against S. frugiperda. The results obtained provide guidelines for the use of wood waste to produce sustainable and effective insecticides in the fight against S. frugiperda. In addition, considering that a phytochemical complex mixture allows the simultaneous activation of different action mechanisms, the development of resistance in insects is slower.
西红花玉桂（Cedrela odorata L.）是楝科植物，主要用于木材生产。尽管西红花玉桂精油（EO）主要含有倍半萜烯，但它的杀虫潜力尚不清楚。EO 的亲脂性和高降解能力限制了其在害虫防治中的应用。然而，目前关于 EO 的纳米乳化的知识，除了可以改善其分散性之外，还可以延长其在田间的持久性。本工作的目的是开发西红花玉桂 EO 的纳米乳液，并评估其对草地贪夜蛾（Spodoptera frugiperda）的杀虫活性。EO 通过西红花玉桂脱水叶片的水蒸馏获得，纳米乳液使用非离子表面活性剂（吐温 80 和司盘 80）通过搅拌和超声分散的组合方法制备。在 5°C 和 25°C 下储存 90 天的情况下，验证了粒径<200nm 的纳米乳液的稳定性。西红花玉桂 EO 及其相应的纳米乳液对 S. frugiperda 均具有致死特性。研究结果为利用木材废料生产可持续和有效的杀虫剂防治 S. frugiperda 提供了指导。此外，由于植物化学复杂混合物可以同时激活不同的作用机制，昆虫对其产生抗性的速度较慢。
