College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China.
Oxid Med Cell Longev. 2018 Mar 25;2018:1538632. doi: 10.1155/2018/1538632. eCollection 2018.
Methanol extracts of L. fruits are extensively studied for their antiaging activities. A new cucurbitane-type triterpenoid (1) and nine other known compounds (2-10) were isolated, and their structures were determined according to their spectroscopic characteristics and chemical derivatization. Biological evaluation was performed on a K6001 yeast bioassay system. The results indicated that all the compounds extended the replicative lifespan of K6001 yeast significantly. Compound 9 was used to investigate the mechanism involved in the increasing of the lifespan. The results indicated that this compound significantly increases the survival rate of yeast under oxidative stress and decreases ROS level. Further study on gene expression analysis showed that compound 9 could reduce the levels of and and increase and gene expression. In addition, it could not extend the lifespan of the yeast mutants of , , , and . These results demonstrate that compound 9 exerts antiaging effects via antioxidative stress and regulation of , , , and yeast gene expression.
该文以 K6001 酵母生物测定系统为平台，对从苦瓜果实甲醇提取物中分离得到的 1 个新的葫芦烷型三萜（1）和 9 个已知化合物（2-10）进行了抗衰老活性筛选。结果表明，所有化合物均能显著延长 K6001 酵母的复制寿命。化合物 9 用于研究延长寿命所涉及的机制。结果表明，该化合物能显著提高酵母在氧化应激下的存活率并降低 ROS 水平。进一步的基因表达分析表明，化合物 9 能降低 和 水平，增加 和 基因表达。此外，它不能延长 的酵母突变体、 、 、和 的寿命。这些结果表明，化合物 9 通过抗氧化应激和调节 、 、 、和 酵母基因表达发挥抗衰老作用。
