Fan Zhiyong, Xiao Yong, Chen Yonghui, Wu Xin, Zhang Guanglei, Wang Qinhua, Xie Chunyan
Engineering Research Center for Feed Safety and Efficient Utilization of Ministry of Education, Institute of Animal Nutrition, Hunan Agricultural University, Changsha 410128, China.
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Anim Nutr. 2015 Dec;1(4):271-275. doi: 10.1016/j.aninu.2015.11.002. Epub 2015 Dec 12.
This study was conducted to investigate the effects of catechins on reproductive performance, antioxidative capacity and immune function of gestating sows. A total of 60 cross-bred (Landrace × Large White) multiparious sows were blocked by body weight, parity and backfact and randomly allocated to 1 of 5 treatments: 0, 100, 200, 300, or 400 mg/kg catechins. Dietary treatments were imposed from mating to d 40 of gestation of sows. At farrowing, litter total born, born alive, dead, and normal-(healthy piglets, ≥0.85 kg) and low-birth weight piglets (<0.85 kg) were recorded. Within 3.00 ± 0.50 days after farrowing litter size was standardized to 8.00 ± 1.50 piglets within treatment. The piglets were weighed at birth (d 1) and weaning (d 28). Sows serum samples were obtained from blood samples collected on d 40 of gestation for analyses of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), hydrogen peroxide (HO), nitric oxide synthetase (NOS) and nitrogen monoxide (NO). Our results showed that supplementation of catechins at levels of 200 or 300 mg/kg led to improvements in litter born alive ( < 0.01) and piglet born healthy ( < 0.01) and a decrease in stillborn ( < 0.05) at farrowing when compared with the control. In comparison with the control, catechins at any supplemental levels all enhanced the serum SOD ( < 0.05) and CAT ( < 0.01) activities of sows at farrowing but no obvious differences in the serum GSH-Px and NOS activities were observed in this trial ( > 0.05). Sows received 200 mg catechin per kg diets showed a reduction ( < 0.05) of the serum MDA level at farrowing compared with all other treatments. Sows received all the levels of catechin showed a reduction ( < 0.05) of serum HO level compared with sows received the control diet on both d 40 of gestation and farrowing. Our results demonstrated that the catechins may be a potential antioxidant to increase the reproductive performance and antioxidative capacity of sows when it was added into diets during the early gestation.
本研究旨在探讨儿茶素对妊娠母猪繁殖性能、抗氧化能力和免疫功能的影响。总共60头杂交（长白猪×大白猪）经产母猪按体重、胎次和背膘厚度进行分组，并随机分配到5种处理中的1种：0、100、200、300或400毫克/千克儿茶素。从母猪配种至妊娠第40天进行日粮处理。在分娩时，记录总产仔数、活产仔数、死胎数，以及正常（健康仔猪，≥0.85千克）和低出生体重仔猪（<0.85千克）的数量。在分娩后3.00±0.50天内，将各处理组的仔猪数量标准化为8.00±1.50头。在出生（第1天）和断奶（第28天）时对仔猪进行称重。在妊娠第40天采集母猪血液样本，获取血清样本，用于分析谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、过氧化氢（HO）、一氧化氮合酶（NOS）和一氧化氮（NO）。我们的结果表明，与对照组相比，添加200或300毫克/千克儿茶素可提高分娩时的活产仔数（P<0.01）和健康仔猪出生数（P<0.01），并降低死胎数（P<0.05）。与对照组相比，任何添加水平的儿茶素均能提高分娩时母猪血清SOD（P<0.05）和CAT（P<0.01）的活性，但在本试验中，血清GSH-Px和NOS活性未观察到明显差异（P>0.05）。与所有其他处理相比，每千克日粮添加200毫克儿茶素的母猪在分娩时血清MDA水平降低（P<0.05）。在妊娠第40天和分娩时，与饲喂对照日粮的母猪相比，所有添加水平儿茶素的母猪血清HO水平均降低（P<0.05）。我们的结果表明，在妊娠早期将儿茶素添加到日粮中时，儿茶素可能是一种潜在的抗氧化剂，可提高母猪的繁殖性能和抗氧化能力。
