Bonazza Veronica, Hajistilly Christopher, Patel Dinker, Patel Jatan, Woo Rita, Cocchi Marco Angelo, Buffoli Barbara, Lancini Davide, Gheno Ezio, Rezzani Rita, Jahanzeb Sadia, Hunnisett Adrian, Almasri Maher, Lucchina Alberta Greco, Brucoli Matteo, Mortellaro Carmen, Rodella Luigi Fabrizio
Department of Clinical and Experimental Sciences, Division of Anatomy and Physiopathology, University of Brescia, Brescia, Italy.
Faculty of Dentistry, BPP University, Birmingham, UK.
J Craniofac Surg. 2018 Nov;29(8):2291-2295. doi: 10.1097/SCS.0000000000004607.
Platelet concentrates represent a new approach to improve tissue regeneration and can be used alone or together with autogenous bone, recombinant human growth factors, and/or other biomaterials, to enhance tissue regeneration. Among platelet concentrates, concentrated growth factors (CGFs) exhibit an interesting clinical and biotechnological application potential.
The aim of this study was to evaluate the in vitro release of 4 growth factors (bone morphogenetic proteins [BMP] -2, BMP-7, transforming growth factor [TGF] -β1, and insulin-like growth factor [IGF] -1) by the enzyme-linked immunosorbent assay (ELISA) technique, in CGFs mixed or not with β-tricalcium phosphate (β-TCP), using or not the Round-up device, at different times.
CGFs were obtained from healthy volunteers, mixed or not with β-TCP, using or not the Round-up device. The release of 4 growth factors from these CGFs was then measured at 5 hours, 1, 3, 6, and 8 days, using the ELISA assay.
Comparison of the results obtained with those achieved for CGFs alone showed that BMP2 and BMP-7 release, significantly increased in CGFs mixed with Round-up and β-TCP, TGF-β1 release was similar to CGFs alone, whereas IG-1 release was lower compared with CGFs alone.
The present data suggest that β-TCP addition to CGF could enhance and improve tissue regeneration, especially bone regeneration, increasing the release of some growth factors that play an important role in osteogenesis.
血小板浓缩物是一种促进组织再生的新方法，可单独使用，也可与自体骨、重组人生长因子和/或其他生物材料联合使用，以增强组织再生。在血小板浓缩物中，浓缩生长因子（CGF）具有有趣的临床和生物技术应用潜力。
本研究旨在通过酶联免疫吸附测定（ELISA）技术，评估在不同时间，使用或不使用Round-up装置、与β-磷酸三钙（β-TCP）混合或未混合的CGF中4种生长因子（骨形态发生蛋白[BMP]-2、BMP-7、转化生长因子[TGF]-β1和胰岛素样生长因子[IGF]-1）的体外释放情况。
从健康志愿者获取CGF，使用或不使用Round-up装置，与β-TCP混合或不混合。然后在5小时、1天、3天、6天和8天，使用ELISA测定法测量这些CGF中4种生长因子的释放情况。
将所得结果与单独CGF的结果进行比较，结果显示，与使用Round-up装置并与β-TCP混合的CGF相比，BMP2和BMP-7的释放显著增加，TGF-β1的释放与单独的CGF相似，而IG-1的释放与单独的CGF相比更低。
目前的数据表明，向CGF中添加β-TCP可增强和改善组织再生，尤其是骨再生，增加一些在骨生成中起重要作用的生长因子的释放。
