de Souza Aline Cristine da Silva, Gonсalves Geferson de Almeida, Soares Andréia Assunсão, de Sá-Nakanishi Anacharis B, de Santi-Rampazzo Ana Paula, Natali Maria Raquel Marсal, Peralta Rosane Marina, Bracht Adelar
Post-graduate Program of Biological Sciences, State University of Maringá, Maringá, PR, Brazil.
Post-graduate Program of Food Science, State University of Maringá, Maringá, PR, Brazil.
Int J Med Mushrooms. 2018;20(2):101-117. doi: 10.1615/IntJMedMushrooms.2018025309.
The purpose of this study was to evaluate possible effects of the administration of an aqueous Agaricus brasiliensis extract on the oxidative state of the liver, brain, and plasma in adjuvant-induced arthritic rats, a model for human rheumatoid arthritis. Daily doses of 400 mg · kg-1 were administered by gavage for 23 days. This treatment produced significant diminutions in protein carbonylation and lipid damage in the liver, brain, and plasma. It also diminished the tissue reactive oxygen species and increased the antioxidant capacity of the plasma. Antioxidant defenses, which are diminished by arthritis, were improved by treatment with the A. brasiliensis extract, as revealed by preservation of the reduced glutathione and protein thiol levels and by the tendency of the activities of some antioxidant enzymes to normalize. The increased glucose-6-phosphate dehydrogenase activity was also almost normalized by the treatment. In addition, there were indications that A. brasiliensis can inhibit the initiation of structural events that can lead to hepatocyte necrosis. In conclusion, A. brasiliensis aqueous preparations can, in principle, be visualized as potential auxiliaries in the treatment of patients with rheumatoid arthritis as a result of their capacity to reduce oxidative stress. This effect was exerted in multiple organs, as can be judged from the results obtained in the liver, brain, and plasma. The continuous ingestion of A. brasiliensis as specific preparations or as a food supplement can possibly help to attenuate the systemic effects of rheumatoid arthritis and improve the quality of life of patients affected by this disease.
本研究的目的是评估给予巴西蘑菇水提取物对佐剂诱导的关节炎大鼠（一种人类类风湿性关节炎模型）肝脏、大脑和血浆氧化状态的可能影响。通过灌胃给予每日剂量400mg·kg-1，持续23天。这种治疗使肝脏、大脑和血浆中的蛋白质羰基化和脂质损伤显著减少。它还减少了组织中的活性氧，并提高了血浆的抗氧化能力。如通过还原型谷胱甘肽和蛋白质巯基水平的保留以及一些抗氧化酶活性趋于正常的趋势所显示的，关节炎导致降低的抗氧化防御通过巴西蘑菇提取物治疗得到改善。治疗还使增加的葡萄糖-6-磷酸脱氢酶活性几乎恢复正常。此外，有迹象表明巴西蘑菇可以抑制可能导致肝细胞坏死的结构事件的起始。总之，由于其降低氧化应激的能力，巴西蘑菇水制剂原则上可被视为类风湿性关节炎患者治疗中的潜在辅助药物。从在肝脏、大脑和血浆中获得的结果可以判断，这种作用在多个器官中发挥。持续摄入巴西蘑菇作为特定制剂或作为食品补充剂可能有助于减轻类风湿性关节炎的全身影响，并改善受该疾病影响患者的生活质量。
