Sims N R, Blass J P
Altschul Laboratory for Dementia Research, Cornell University Medical College, White Plains, New York 10605.
Metab Brain Dis. 1986 Mar;1(1):83-90. doi: 10.1007/BF00998479.
The activity of the enzyme phosphofructokinase (PFK) was comparable in cultured skin fibroblasts from eight patients with Alzheimer's disease and eight age- and sex-matched controls. Mean activities were similar in the two groups whether measured under nonallosteric conditions at pH 8.0 or under allosteric conditions at pH 7.0, in the presence of 0.1 or 1 mM ATP. Activities of PFK in Alzheimer's disease and control cells also showed a similar temperature dependence and similar isozyme patterns on column chromatography. These results argue against the existence of significant structural variations of PFK in Alzheimer's disease.
在来自8名阿尔茨海默病患者和8名年龄及性别匹配的对照者的培养皮肤成纤维细胞中，磷酸果糖激酶（PFK）的活性相当。无论是在pH 8.0的非别构条件下，还是在pH 7.0、存在0.1 mM或1 mM ATP的别构条件下进行测量，两组的平均活性都相似。阿尔茨海默病患者和对照细胞中PFK的活性在温度依赖性以及柱色谱上的同工酶模式方面也表现出相似性。这些结果表明阿尔茨海默病中不存在PFK的显著结构变异。
