MRC Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, EH16 4UU Edinburgh, UK
Trinity Biomedical Sciences Institute, Trinity College Dublin, 152-160 Pearse St, Dublin 2, Dublin, Republic of Ireland.
Philos Trans R Soc Lond B Biol Sci. 2018 Jul 5;373(1750). doi: 10.1098/rstb.2017.0212.
Human pluripotent stem cells (PSCs) offer a scalable alternative to primary and transformed human tissue. PSCs include human embryonic stem cells, derived from the inner cell mass of blastocysts unsuitable for human implantation; and induced PSCs, generated by the reprogramming of somatic cells. Both cell types display the ability to self-renew and retain pluripotency, promising an unlimited supply of human somatic cells for biomedical application. A distinct advantage of using PSCs is the ability to select for genetic background, promising personalized modelling of human biology 'in a dish' or immune-matched cell-based therapies for the clinic. This special issue will guide the reader through stem cell self-renewal, pluripotency and differentiation. The first articles focus on improving cell fidelity, understanding the innate immune system and the importance of materials chemistry, biofabrication and bioengineering. These are followed by articles that focus on industrial application, commercialization and label-free assessment of tissue formation. The special issue concludes with an article discussing human liver cell-based therapies past, present and future.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.
人类多能干细胞（PSCs）为原发性和转化性人体组织提供了一种可扩展的替代方法。PSCs 包括人类胚胎干细胞，来源于不适宜人类植入的囊胚内细胞团；以及通过体细胞重编程产生的诱导多能干细胞。这两种细胞类型都具有自我更新和保持多能性的能力，有望为生物医学应用提供无限供应的人类体细胞。使用 PSCs 的一个明显优势是能够选择遗传背景，有望在“体外”对人类生物学进行个性化建模，或为临床提供免疫匹配的基于细胞的治疗方法。本期特刊将引导读者了解干细胞自我更新、多能性和分化。前几篇文章重点介绍了提高细胞保真度、了解固有免疫系统以及材料化学、生物制造和生物工程的重要性。随后的文章则侧重于工业应用、商业化和无标记组织形成评估。本期特刊以一篇讨论过去、现在和未来基于人类肝细胞的治疗方法的文章结束。
这篇文章是主题特刊“设计人类组织：即将在您附近的实验室出现”的一部分。
