State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming 650091, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan University, Kunming 650091, PR China.
Fitoterapia. 2018 Jul;128:213-217. doi: 10.1016/j.fitote.2018.05.026. Epub 2018 May 22.
The mushroom Stereum hirsutum is parasitized by Tremella aurantia to form a heterogeneous basidiocarp Jin'er, which has been used as food and folk medicine in Chinese society. In present work, the S. hirsutum was fermented in YMG broth, and four novel mixed terpenes, stereumamides A-D (1-4), which are sesquiterpenes combined with α-amino acids to form quaternary ammonium hybrids, were isolated from the Stereum hirsutum FP-91666 and their structures were elucidated by spectroscopic data analysis. Stereumamides A and D showed antibacterial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium, with the minimum inhibitory concentration (MIC) values of 12.5-25.0 μg/mL. The stereumamides A-D should be apparently the first example of naturally occurring a quaternary ammonium compound (QAC) conjugated by sesquiterpene with an α-amino acid. QAC is a common antibacterial agent in food industry, which is found in the mycelium of Stereum hirsutum would suggest that the complex basidiocarp is a functional food and veritable folk medicine.
金耳（Tremella aurantia）寄生于毛栓菌（Stereum hirsutum）形成异层型担子果，在中国社会中被用作食物和民间药物。在本工作中，从毛栓菌 FP-91666 发酵液中分离得到四个新的混合三萜化合物，立体酰胺 A-D（1-4），它们是由α-氨基酸与倍半萜结合形成的季铵盐杂合化合物，并通过光谱数据分析阐明了它们的结构。立体酰胺 A 和 D 对大肠杆菌、金黄色葡萄球菌和鼠伤寒沙门氏菌具有抗菌活性，最低抑菌浓度（MIC）值为 12.5-25.0μg/mL。立体酰胺 A-D 应该是第一个由倍半萜与α-氨基酸结合形成的季铵化合物（QAC）的天然存在的例子。QAC 是食品工业中常见的抗菌剂，在毛栓菌的菌丝体中也有发现，这表明这种复杂的担子果是一种功能性食品和名副其实的民间药物。
