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不同培养条件下的活性与代谢组学

Activities and metabolomics of under different culture conditions.

作者信息

Qu Shuai-Ling, Xie Juan, Wang Jun-Tao, Li Guo-Hong, Pan Xue-Rong, Zhao Pei-Ji

机构信息

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming, Yunnan, China.

出版信息

Front Microbiol. 2023 Jan 12;13:1076577. doi: 10.3389/fmicb.2022.1076577. eCollection 2022.

DOI:10.3389/fmicb.2022.1076577
PMID:36713217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9878563/
Abstract

Many active metabolites have been identified from various species of the fungal genus . A predominant species of this genus is , but there are limited reports on the active ingredients from this species. This study aimed to conduct activity assays and metabolome analysis on extracts of obtained under different culture conditions. Five different solid media were selected to culture the mycelium of and the metabolites were extracted with organic solvents; concurrently, the wild stroma and host complexes of were extracted by ethyl acetate. Extracts were subsequently assayed for various biological activities and were analyzed by untargeted metabolomics. There were significant differences in the activities and metabolites of extracts from different culture conditions and from wild stroma and host complexes. The extracts of stroma and host complexes and mycelia cultured on WGA medium for 21 days exhibited similar effective inhibitory activity against five cell lines. A total of 51 metabolites were annotated and included various structural types. The literatures indicate that most of the identified compounds have a variety of different biological activities. These findings provide the basis for further systematic excavation of and improved utilization of this fungal species.

摘要

已从该真菌属的多个物种中鉴定出许多活性代谢产物。该属的一个主要物种是 ，但关于该物种活性成分的报道有限。本研究旨在对在不同培养条件下获得的 的提取物进行活性测定和代谢组分析。选择五种不同的固体培养基来培养 的菌丝体，并用有机溶剂提取代谢产物；同时，用乙酸乙酯提取 的野生子座和寄主复合体。随后对提取物进行各种生物活性测定，并通过非靶向代谢组学进行分析。不同培养条件下以及野生子座和寄主复合体的 提取物在活性和代谢产物方面存在显著差异。在WGA培养基上培养21天的子座和寄主复合体以及菌丝体的提取物对五种细胞系表现出相似的有效抑制活性。总共注释了51种代谢产物，包括各种结构类型。文献表明，大多数已鉴定的化合物具有多种不同的生物活性。这些发现为进一步系统挖掘 以及更好地利用这种真菌物种提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/41717faa6840/fmicb-13-1076577-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/1bb67ce230d3/fmicb-13-1076577-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/b0e6486000f2/fmicb-13-1076577-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/861125ce970c/fmicb-13-1076577-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/41717faa6840/fmicb-13-1076577-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/1bb67ce230d3/fmicb-13-1076577-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/b0e6486000f2/fmicb-13-1076577-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/861125ce970c/fmicb-13-1076577-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c2b9/9878563/41717faa6840/fmicb-13-1076577-g004.jpg

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