研究在医院发生腺病毒暴发后，含酒精溶液对腺病毒 8、19 和 37 型（引起流行性角膜结膜炎的常见病因）的疗效。
Investigation of the efficacy of alcohol-based solutions on adenovirus serotypes 8, 19 and 37, common causes of epidemic keratoconjunctivitis, after an adenovirus outbreak in hospital.
机构信息
Department of Medical Microbiology, Institute of Health Sciences, Kocaeli University, Kocaeli, Turkey.
Department of Medical Microbiology, Faculty of Medicine, Kocaeli University, Kocaeli, Turkey.
出版信息
J Hosp Infect. 2018 Nov;100(3):e30-e36. doi: 10.1016/j.jhin.2018.05.011. Epub 2018 May 21.
BACKGROUND
Hand antiseptics are of great importance in the prevention of nosocomial infections. Due to the frequent occurrence of adenoviral epidemic keratoconjunctivitis outbreaks in the study hospital, it was necessary to investigate the efficiency of alcohol-based solutions on adenoviruses.
AIM
To investigate the efficacy of alcohol-based solutions commonly found in hand antiseptics against adenovirus serotypes 8, 19 and 37.
METHODS
The efficacy of ethanol, isopropanol, chlorhexidine-digluconate, n-butanol and different combinations of these antiseptics on adenovirus serotypes that typically cause epidemic keratoconjunctivitis was investigated. The effect of antiseptic substances was investigated using a quantitative suspension test technique according to EN-14476. Antiseptics were prepared as follows: 70% ethanol, 70% isopropanol, 70% ethanol+0.5% chlorhexidine-digluconate, 70% isopropanol+0.5% chlorhexidine-digluconate, 60% ethanol+10% isopropanol, 60% ethanol+10% isopropanol+0.5% chlorhexidine-digluconate and 60% ethanol+10% isopropanol+1% n-butanol. The effect of antiseptics was evaluated at 30, 60 and 120 s.
FINDINGS
The highest reduction in virus titre was detected with 60% ethanol+10% isopropanol+1% n-butanol. This caused a reduction of 2.5log, 3 log and 2.5log in adenovirus serotypes 8, 19 and 37, respectively. The lowest reduction in virus titre was detected with 70% ethanol+0.5% chlorhexidine-digluconate and 70% isopropanol+0.5% chlorhexidine-digluconate. These combinations caused a reduction of 1.62log in adenovirus serotypes 19 and 37. All antiseptics showed efficacy levels below 4log which is regarded as the efficiency limit by EN-14476.
CONCLUSION
The alcohol-based solutions tested in this study were not found to be sufficiently effective against adenovirus serotypes. Further studies to investigate the efficiency of different alcohol-based solutions are required.
背景
手部消毒剂在预防医院感染方面非常重要。由于研究医院经常发生腺病毒流行的角膜结膜炎暴发，因此需要研究醇基溶液对腺病毒的效果。
目的
研究常见的手部消毒剂中对腺病毒血清型 8、19 和 37 的效果。
方法
采用定量悬浮试验技术（根据 EN-14476 进行），研究乙醇、异丙醇、葡萄糖酸氯己定、正丁醇以及这些消毒剂的不同组合对引起流行角膜结膜炎的典型腺病毒血清型的效果。使用以下消毒剂制备物研究消毒剂物质的作用：70%乙醇、70%异丙醇、70%乙醇+0.5%葡萄糖酸氯己定、70%异丙醇+0.5%葡萄糖酸氯己定、60%乙醇+10%异丙醇、60%乙醇+10%异丙醇+0.5%葡萄糖酸氯己定和 60%乙醇+10%异丙醇+1%正丁醇。在 30、60 和 120s 时评估消毒剂的效果。
结果
在 60%乙醇+10%异丙醇+1%正丁醇中检测到病毒滴度的最大降低。这导致腺病毒血清型 8、19 和 37 的病毒滴度分别降低 2.5log、3log 和 2.5log。在 70%乙醇+0.5%葡萄糖酸氯己定和 70%异丙醇+0.5%葡萄糖酸氯己定中检测到病毒滴度的最低降低。这两种组合导致腺病毒血清型 19 和 37 的病毒滴度降低 1.62log。所有消毒剂的效果水平均低于 4log，根据 EN-14476，这被视为效率限制。
结论
本研究中测试的醇基溶液对腺病毒血清型的效果并不理想。需要进一步研究不同醇基溶液的效率。
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