MAPKK 家族成员对棉花黄萎病抗性的精细调控。
Subtle regulation of cotton resistance to Verticillium wilt mediated by MAPKK family members.
机构信息
The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuan Ming Yuan Road, Beijing 100193, PR China; College of Life Science, Shanxi Normal University, No.1 GongYuan Street, Linfen, Shanxi 041004, PR China.
The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2, West Yuan Ming Yuan Road, Beijing 100193, PR China.
出版信息
Plant Sci. 2018 Jul;272:235-242. doi: 10.1016/j.plantsci.2018.05.003. Epub 2018 May 8.
Verticillium wilt caused by soil-borne fungus of Verticillium dahliae Kleb. is one of the most devastating diseases of cotton. Since the hierarchically organized mitogen-activated protein kinase (MAPK) cascade plays pivotal roles in signaling plant defense against pathogen attack, and the key nodes of MAPKKs (MKKs) may serve as for the convergence and divergence of signals in MAPK cascades, the possible relations between MAPK signaling and cotton Verticillium resistance were examined in this study. A total of 24 MKK genes were identified in the Gossypium hirsutum L. genome and then classified based on phylogenetic analysis. Then the regulation roles of all types of cotton MKKs in activation of cotton disease resistance were tested with the virus-induced gene silencing (VIGS) method. The results showed that three types of MKKs (GhMKK4, GhMKK6 and GhMKK9) positively regulate, while GhMKK10 negatively regulate the cotton resistance to Verticillium wilt. Further, more subtle regulation of cotton resistance mediated by MKK genes were revealed. In GhMKK9, only Gh_A12G2448 and Gh_D12G2574 displayed positive regulation of cotton resistance; whereas only Gh_A12G1883 and Gh_D12G2062 displayed negative regulation of cotton resistance in GhMKK10. All these results show that MKK members in MAPK signal cascades play dual roles in subtly regulating of cotton resistance to Verticillium wilt.
由土壤传播的真菌黄萎轮枝菌引起的黄萎病是棉花最具破坏性的疾病之一。由于层次组织的丝裂原活化蛋白激酶（MAPK）级联在植物防御病原体攻击的信号转导中起着关键作用，并且 MAPKKs（MKKs）的关键节点可能作为 MAPK 级联中信号的收敛和发散，因此本研究检查了 MAPK 信号与棉花黄萎病抗性之间的可能关系。在陆地棉基因组中鉴定了 24 个 MKK 基因，并根据系统发育分析进行了分类。然后，利用病毒诱导的基因沉默（VIGS）方法测试了所有类型的棉花 MKK 对棉花抗病性的激活的调节作用。结果表明，三种类型的 MKK（GhMKK4、GhMKK6 和 GhMKK9）正向调节，而 GhMKK10 负向调节棉花对黄萎病的抗性。此外，还揭示了 MKK 基因介导的棉花抗性的更细微调节。在 GhMKK9 中，只有 Gh_A12G2448 和 Gh_D12G2574 显示对棉花抗性的正向调节；而在 GhMKK10 中，只有 Gh_A12G1883 和 Gh_D12G2062 显示对棉花抗性的负向调节。所有这些结果表明，MAPK 信号级联中的 MKK 成员在细微调节棉花对黄萎病的抗性中起双重作用。
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