van der Merwe D G, Sellschop J P, Erasmus C S, Pettifor J M
WITS-CSIR Schonland Research Centre for Nuclear Sciences, University of the Witwatersrand, Republic of South Africa.
J Trace Elem Electrolytes Health Dis. 1988 Dec;2(4):209-14.
A tracer technique using the stable isotope calcium-48 was developed to facilitate studies of human calcium metabolism. Small samples of 1 ml serum were used. Calcium levels were determined with good precision using neutron-activation analysis after pre-concentration of the serum calcium by oxalate precipitation. The method has sufficient sensitivity to detect induced dilutions of the tracer by administering other less costly isotopes of calcium. The ability to utilize small volumes of sample is of direct benefit to paediatric metabolism research. The favourable results obtained for two calcium loading tests demonstrate the applicability of the technique to intestinal absorption studies.
一种使用稳定同位素钙-48的示踪技术被开发出来，以促进对人体钙代谢的研究。使用了1毫升血清的小样本。通过草酸盐沉淀对血清钙进行预浓缩后，采用中子活化分析测定钙水平，测定精度良好。该方法具有足够的灵敏度，能够通过施用其他成本较低的钙同位素来检测示踪剂的诱导稀释。利用少量样本的能力对儿科代谢研究有直接益处。两项钙负荷试验获得的良好结果证明了该技术在肠道吸收研究中的适用性。
