Dept of Obstetrics and Gynecology, the Second Peoples Hospital of Hefei, Affiliated Hefei Hospital of Anhui Medical University, Hefei, 230011, China.
Department of Pharmacology, Basic Medical College, Anhui Medical University, Hefei, 230032, China.
Phytother Res. 2018 Sep;32(9):1836-1847. doi: 10.1002/ptr.6119. Epub 2018 May 31.
The aim of this study was to investigate the effects of Grape Seed Proanthocyanidins (GSP) on Nω-Nitro-L-arginine methyl ester-induced hypertension in pregnant mice. Fifty Kunming mice were randomized into control, control + GSP, model, and model + GSP. Three weeks later, the artery systolic blood pressure was examined and the related pathological changes were detected. Aorta relaxation function was assessed by aorta ring apparatus. Blood urea nitrogen and serum creatinine were measured by an automatic biochemistry analyzer. Colorimetric analysis, enzyme-linked immunosorbent assay, immunofluorescence, and western blot were applied to detect related indicator in serum, cardiac, and kidney tissues. The results showed that GSP treatment for 3 weeks could improve cardiovascular and kidney remodeling indexes and decrease blood urea nitrogen and serum creatinine content in serum, as well as could ameliorate oxidative stress status and endothelial dysfunction. Therefore, it is for the first time found that GSP exerts protective effect against Nω-Nitro-L-arginine methyl ester-induced hypertension in pregnant mice, which provided a theoretical basis for potential application in the clinic.
本研究旨在探讨葡萄籽原花青素（GSP）对 Nω-硝基-L-精氨酸甲酯诱导的妊娠高血压小鼠的影响。将 50 只昆明小鼠随机分为对照组、对照组+GSP、模型组和模型组+GSP。3 周后，检测动脉收缩压，并检测相关的病理变化。采用主动脉环装置评估主动脉松弛功能。采用自动生化分析仪检测血尿素氮和血清肌酐。应用比色分析、酶联免疫吸附试验、免疫荧光和 Western blot 检测血清、心脏和肾脏组织中的相关指标。结果表明，GSP 治疗 3 周可改善心血管和肾脏重塑指标，降低血清中血尿素氮和血清肌酐含量，并改善氧化应激状态和内皮功能障碍。因此，首次发现 GSP 对 Nω-硝基-L-精氨酸甲酯诱导的妊娠高血压小鼠具有保护作用，为其在临床上的潜在应用提供了理论依据。
