Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
School of Life Sciences, Northeast Normal University, Changchun 130022, China.
Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:168-179. doi: 10.1016/j.msec.2018.04.061. Epub 2018 Apr 22.
A novel biodegradable conducting polymer, PLA-b-AP-b-PLA (PAP) triblock copolymer of poly (l-lactide) (PLA) and aniline pentamer (AP) with electroactivity and biodegradability, was synthesized and its potential application in cardiac tissue engineering was studied. The PAP copolymer presented better biocompatibility compared to PANi and PLA because of promoted cell adhesion and spreading of rat cardiac myoblasts (H9c2 cell line) on PAP/PLA thin film. After pulse electrical stimulation (5 V, 1 Hz, 500 ms) for 6 days, the proliferation ratio, and intracellular calcium concentration of H9c2 cells on PAP/PLA were improved significantly. Meanwhile, cell morphology changed by varying the pulse electrical signals. Especially, the oriented pseudopodia-like structure was observed from H9c2 cells on PAP/PLA after electrical stimulation. It is regarded that the novel conducting copolymer could enhance electronic signals transferring between cells because of its special electrochemical properties, which may result in the differentiation of cardiac myoblasts.
一种新型可生物降解导电聚合物 PLA-b-AP-b-PLA（PAP），由聚（L-乳酸）（PLA）和苯胺五聚体（AP）组成，具有电活性和可生物降解性，被合成出来，并研究了其在心脏组织工程中的潜在应用。与 PANi 和 PLA 相比，PAP 共聚物由于促进了大鼠心肌细胞（H9c2 细胞系）在 PAP/PLA 薄膜上的黏附和铺展，表现出更好的生物相容性。经过 6 天的脉冲电刺激（5V、1Hz、500ms）后，H9c2 细胞在 PAP/PLA 上的增殖率和细胞内钙离子浓度显著提高。同时，细胞形态也随着脉冲电信号的变化而变化。特别是，在电刺激后，从 H9c2 细胞上可以观察到 PAP/PLA 上定向的伪足样结构。由于其特殊的电化学性质，新型导电共聚物可以增强细胞间的电子信号传递，从而可能导致心肌细胞的分化。
