Dougnon Tamègnon Victorien, Hounsa Edna, Agbodjento Eric, Koudokpon Hornel, Legba Boris, Fabiyi Kafayath, Afaton Anny, Sintondji Kévin, Akpode Benoît, Klotoé Jean Robert, Tchobo Fidèle, Bankole Honoré, Dougnon Tossou Jacques
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Laboratory of Research in Applied Biology, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin.
Training and Research Laboratory in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Abomey-Calavi, Benin.
Evid Based Complement Alternat Med. 2021 Apr 28;2021:6676904. doi: 10.1155/2021/6676904. eCollection 2021.
The use of medicinal plants in traditional medicine is a common practice in developing countries. However, this unregulated or poorly rational use may present a dose-dependent risk of toxicity to humans. This study aimed to explore the phytochemical and toxicological characteristics of ten (10) plant species used in the traditional treatment of infectious diarrhea in Benin. The acute toxicity of aqueous and hydroethanolic extracts of , , , , , , , and was evaluated following the OECD 423 protocol at a single dose of 2000 mg/kg. This safety test was complemented by a larval cytotoxicity test. Hematological and biochemical examinations, as well as a histological study of the liver and kidneys, were performed. Larval cytotoxicity was assessed by the sensitivity of larvae to different concentrations of the plant extracts studied. Testing for chemical compounds was performed on the basis of differential staining and precipitation reactions. The mean lethal concentration (LC) was determined by the probit method. The qualitative phytochemical screening of the plants studied revealed the presence of catechic tannins, gallic tannins, flavonoids, anthocyanins and sterol-terpenes, alkaloids, saponosides, and reducing compounds. This composition varied according to the plants studied. Acute toxicity data indicated that there was no mortality and no structural and functional alterations of the liver and kidneys of treated animals. Larval cytotoxicity data suggest that the plants studied are not cytotoxic (LC 0.1 mg/mL). These observations reflect the safety of these plants and justify their use in traditional medicine in the treatment of many diseases including diarrheal diseases.
在发展中国家，传统医学中使用药用植物是一种常见做法。然而，这种无监管或不合理的使用可能会给人类带来剂量依赖性的毒性风险。本研究旨在探究贝宁用于传统治疗感染性腹泻的10种植物的植物化学和毒理学特征。按照经合组织423协议，以2000毫克/千克的单剂量评估了[植物名称1]、[植物名称2]、[植物名称3]、[植物名称4]、[植物名称5]、[植物名称6]、[植物名称7]、[植物名称8]和[植物名称9]水提取物和水乙醇提取物的急性毒性。这项安全性测试通过幼虫细胞毒性试验得到补充。进行了血液学和生化检查以及肝脏和肾脏的组织学研究。通过[幼虫名称]幼虫对所研究植物提取物不同浓度的敏感性来评估幼虫细胞毒性。基于鉴别染色和沉淀反应对化合物进行检测。采用概率单位法确定平均致死浓度（LC）。对所研究植物进行的定性植物化学筛选显示存在儿茶酚单宁、没食子单宁、黄酮类化合物、花青素和甾醇 - 萜类、生物碱、皂苷以及还原性化合物。这种成分因所研究的植物而异。急性毒性数据表明，处理过的动物没有死亡，肝脏和肾脏也没有结构和功能改变。幼虫细胞毒性数据表明所研究的植物没有细胞毒性（LC＞0.1毫克/毫升）。这些观察结果反映了这些植物具有安全性，并证明它们在传统医学中用于治疗包括腹泻病在内的多种疾病是合理的。
