白花菜科植物刺山柑茎皮提取物对戊四氮诱导的大鼠氧化应激和癫痫发作具有保护作用。
DC. (Capparaceae) Stem Bark Extract Protects against Pentylenetetrazole-Induced Oxidative Stress and Seizures in Rats.
作者信息
Benneh Charles Kwaku, Biney Robert Peter, Tandoh Augustine, Ampadu Felix Agyei, Adongo Donatus Wewura, Jato Jonathan, Woode Eric
机构信息
Department of Pharmacology and Toxicology, School of Pharmacy, University of Health and Allied Sciences, Ho, Ghana.
Department of Pharmacology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana.
出版信息
Evid Based Complement Alternat Med. 2018 May 2;2018:9684138. doi: 10.1155/2018/9684138. eCollection 2018.
INTRODUCTION
The stem bark of DC. (Capparaceae) is traditionally used for management of epilepsy. Our aim was to evaluate the antiseizure potential and identify possible mechanisms by which the effects are registered.
METHODS
The petroleum ether/ethyl acetate extract (100-1000 mg kg) was administered per os to male Sprague-Dawley rats after pretreatment with flumazenil (0.3 mg kg) or L-arginine (150 mg kg) or sildenafil (5 mg kg) and they subsequently received a subcutaneous injection of pentylenetetrazole (65 mg kg). Rats were observed for latency to and duration of myoclonic seizures and additionally the level of protection against oxidant markers and products was assessed and .
RESULTS
The extract (300 and 1000 mg kg, p.o.) significantly delayed the onset and decreased the duration and frequency of PTZ-induced convulsions. The anticonvulsant effect of MAE (300 mg kg, p.o.) was reversed by pretreatment with flumazenil, L-arginine, or sildenafil. Also, MAE (300 mg kg) treatment reversed significantly PTZ-induced oxidative stress in rat brain tissue.
CONCLUSION
The petroleum ether/ethyl acetate fraction exhibits antiseizure activity by affecting GABAergic and nitric oxide-cGMP pathways. In addition, the extract protects against the generation of free radicals and the oxidative products of the PTZ-induced seizures.
引言
DC.（十字花科）的茎皮传统上用于治疗癫痫。我们的目的是评估其抗癫痫潜力，并确定产生这些作用的可能机制。
方法
在分别用氟马西尼（0.3 mg/kg）或L-精氨酸（150 mg/kg）或西地那非（5 mg/kg）预处理后，将石油醚/乙酸乙酯提取物（100 - 1000 mg/kg）经口给予雄性Sprague-Dawley大鼠，随后给它们皮下注射戊四氮（65 mg/kg）。观察大鼠肌阵挛性发作的潜伏期和持续时间，并评估其对氧化标志物和产物的保护水平。
结果
提取物（300和1000 mg/kg，经口）显著延迟了发作起始，并降低了戊四氮诱导惊厥的持续时间和频率。氟马西尼、L-精氨酸或西地那非预处理可逆转MAE（300 mg/kg，经口）的抗惊厥作用。此外，MAE（300 mg/kg）处理可显著逆转戊四氮诱导的大鼠脑组织氧化应激。
结论
石油醚/乙酸乙酯部分通过影响γ-氨基丁酸能和一氧化氮 - 环鸟苷酸途径表现出抗癫痫活性。此外，该提取物可防止自由基的产生以及戊四氮诱导癫痫发作的氧化产物。
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