Instituto Universitario de Investigación CIBIO (Centro Iberoamericano de la Biodiversidad), University of Alicante, Carretera de San Vicente del Raspeig, s/n, San Vicente del Raspeig, Alicante, Spain.
Genomics and Proteomics Unit, Servicios Técnicos de Investigación, University of Alicante, Carretera de San Vicente del Raspeig, s/n, San Vicente del Raspeig, Alicante, Spain.
PLoS One. 2018 Jun 1;13(6):e0198093. doi: 10.1371/journal.pone.0198093. eCollection 2018.
Amino acids play a central role in aphid-plant interactions. They are essential components of plant primary metabolism, function as precursors for the synthesis of defense-related specialized metabolites, and are major growth-limiting nutrients for aphids. To quantify changes in the free amino acid content of pepper (Capsicum annuum L.) leaves in response to green peach aphid (Myzus persicae Sulzer) feeding, plants were infested with a low (20 aphids/plant) or a high (200 aphids/plant) aphid density in time-course experiments ranging from 3 hours to 7 days. A parallel experiment was conducted with pepper plants that had been subjected to water stress. Factor Analysis of Mixed Data revealed a significant interaction of time x density in the free amino acid response of aphid-infested leaves. At low aphid density, M. persicae did not trigger a strong response in pepper leaves. Conversely, at high density, a large increase in total free amino acid content was observed and specific amino acids peaked at different times post-infestation. Comparing aphid-infested with water-stressed plants, most of the observed differences were quantitative. In particular, proline and hydroxyproline accumulated dramatically in response to water stress, but not in response to aphid infestation. Some additional differences and commonalities between the two stress treatments are discussed.
氨基酸在蚜虫-植物相互作用中起着核心作用。它们是植物初级代谢的重要组成部分，作为防御相关特殊代谢物合成的前体，也是蚜虫生长的主要限制养分。为了量化辣椒（Capsicum annuum L.）叶片对绿桃蚜（Myzus persicae Sulzer）取食的游离氨基酸含量的变化，在从 3 小时到 7 天的时间过程实验中，用低（20 头/株）或高（200 头/株）蚜虫密度对植物进行了侵染。与受到水分胁迫的辣椒植株进行了平行实验。混合数据的因子分析显示，受蚜虫侵染的叶片中游离氨基酸的响应存在时间 x 密度的显著相互作用。在低蚜虫密度下，桃蚜不会引发辣椒叶片的强烈反应。相反，在高密度下，总游离氨基酸含量大幅增加，并且特定氨基酸在侵染后不同时间达到峰值。将受蚜虫侵染的植物与受到水分胁迫的植物进行比较，观察到的大多数差异是定量的。特别是脯氨酸和羟脯氨酸在受到水分胁迫时会大量积累，但在受到蚜虫侵染时不会。还讨论了两种胁迫处理之间的一些其他差异和共同点。
