Institute of Pesticide and Environmental Toxicology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
J Hazard Mater. 2018 Sep 5;357:53-62. doi: 10.1016/j.jhazmat.2018.05.066. Epub 2018 May 29.
The dissemination of antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and antibiotic-resistant HPB (ARHPB) from animal feedlot to nearby environment poses a potentially high risk to environmental ecology and public health. Here, a metagenomic analysis was employed to explore the dissemination of ARGs, HPB, and ARHPB from a pig feedlot to surrounding stream and agricultural soils. In total, not detectable (ND)-1,628.4 μg/kg of antibiotic residues, 18 types of ARGs, 48 HPB species, and 216 ARB isolates were detected in all samples. Antibiotic residues from pig feedlot mainly migrated into stream sediments and greenhouse soil. The dominant ARGs and HPB species from pig feedlot spread into stream sediments (tetracycline resistance genes, Clostridium difficile, and Mycobacterium tuberculosis), stream water (multidrug resistance (MDR) genes, Shigella flexneri, and Bordetella pertussis), and greenhouse soil (MDR genes, Bacillus anthracis, and Brucella melitensis). It is concerning that 54.4% of 216 ARB isolates from all samples were potential ARHPB species, and genome sequencing and functional annotation of 4 MDR HPB isolates showed 9 ARG types. Our findings revealed the potential migration and dissemination of antibiotic residues, ARGs, HPB, and ARHPB from pig feedlot to surrounding stream and agricultural soils via pig sewage discharge and manure fertilization.
抗生素耐药基因（ARGs）、人类致病菌（HPB）和抗生素耐药的 HPB（ARHPB）从动物饲养场传播到附近环境，对环境生态和公共健康构成了潜在的高风险。在这里，采用宏基因组分析来探索 ARGs、HPB 和 ARHPB 从猪饲养场到周围溪流和农业土壤的传播。在所有样本中，总共检测到不可检测（ND）-1628.4μg/kg 的抗生素残留、18 种 ARGs、48 种 HPB 物种和 216 株 ARB 分离株。猪饲养场的抗生素残留主要迁移到溪流沉积物和温室土壤中。来自猪饲养场的主要 ARGs 和 HPB 物种传播到溪流沉积物（四环素耐药基因、艰难梭菌和结核分枝杆菌）、溪流水（多药耐药基因、福氏志贺菌和百日咳博德特氏菌）和温室土壤（多药耐药基因、炭疽芽孢杆菌和布鲁氏菌）。令人担忧的是，所有样本中 216 株 ARB 分离株中有 54.4%是潜在的 ARHPB 物种，对 4 株 MDR HPB 分离株的基因组测序和功能注释显示了 9 种 ARG 类型。我们的研究结果表明，抗生素残留、ARGs、HPB 和 ARHPB 可能通过猪污水排放和粪便施肥从猪饲养场传播到周围溪流和农业土壤。
