Ganogpichayagrai Aunyachulee, Palanuvej Chanida, Ruangrungsi Nijsiri
Public Health Sciences Programme, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Public Health Sciences Programme, College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand.
J Adv Pharm Technol Res. 2017 Jan-Mar;8(1):19-24. doi: 10.4103/2231-4040.197371.
Diabetes and cancer are a major global public health problem. Plant-derived agents with undesirable side-effects were required. This study aimed to evaluate antidiabetic and anticancer activities of the ethanolic leaf extract of cv. Okrong and its active phytochemical compound, mangiferin. Antidiabetic activities against yeast α-glucosidase and rat intestinal α-glucosidase were determined using 1 mM of p-nitro phenyl-α-D-glucopyranoside as substrate. Inhibitory activity against porcine pancreatic α-amylase was performed using 1 mM of 2-chloro-4 nitrophenol-α-D-maltotroside-3 as substrate. Nitrophenol product was spectrophotometrically measured at 405 nm. Anticancer activity was evaluated against five human cancer cell lines compared to two human normal cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Mango leaf extract and mangiferin exhibited dose-dependent inhibition against yeast α-glucosidase with the IC of 0.0503 and 0.5813 mg/ml, respectively, against rat α-glucosidase with the IC of 1.4528 and 0.4333 mg/ml, respectively, compared to acarbose with the IC of 11.9285 and 0.4493 mg/ml, respectively. For anticancer activity, mango leaf extract, at ≥200 μg/ml showed cytotoxic potential against all tested cancer cell lines. In conclusion, mango leaf possessed antidiabetic and anticancer potential .
糖尿病和癌症是全球主要的公共卫生问题。需要具有不良副作用的植物源药剂。本研究旨在评估 cv. Okrong 乙醇叶提取物及其活性植物化学化合物芒果苷的抗糖尿病和抗癌活性。以 1 mM 对硝基苯基-α-D-吡喃葡萄糖苷为底物，测定对酵母α-葡萄糖苷酶和大鼠肠道α-葡萄糖苷酶的抗糖尿病活性。以 1 mM 2-氯-4-硝基苯酚-α-D-麦芽三糖苷-3 为底物，测定对猪胰α-淀粉酶的抑制活性。在 405 nm 处用分光光度法测定硝基苯酚产物。与两种人类正常细胞系相比，使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法评估对五种人类癌细胞系的抗癌活性。芒果叶提取物和芒果苷对酵母α-葡萄糖苷酶表现出剂量依赖性抑制，IC 分别为 0.0503 和 0.5813 mg/ml，对大鼠α-葡萄糖苷酶的 IC 分别为 1.4528 和 0.4333 mg/ml，而阿卡波糖对酵母α-葡萄糖苷酶和大鼠α-葡萄糖苷酶的 IC 分别为 11.9285 和 0.4493 mg/ml。对于抗癌活性，芒果叶提取物在≥200 μg/ml 时对所有测试的癌细胞系均显示出细胞毒性潜力。总之，芒果叶具有抗糖尿病和抗癌潜力。
