Kim Jiyong, Kim Hwan D, Park Jungha, Lee Eun-Seo, Kim Eugene, Lee Seunghun S, Yang Jin-Kyung, Lee Yoon-Sik, Hwang Nathaniel S
School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-742 Republic of Korea.
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 152-742 Republic of Korea.
Biomater Res. 2018 Jan 2;22:1. doi: 10.1186/s40824-017-0112-8. eCollection 2018.
Tissue engineering is an interdisciplinary field that attempts to restore or regenerate tissues and organs through biomimetic fabrication of scaffolds with specific functionality. In recent years, graphene oxide (GO) is considered as promising biomaterial due to its nontoxicity, high dispersity, and hydrophilic interaction, and these characteristics are key to stimulating the interactions between substrates and cells.
In this study, GO substrates were fabricated via chemically immobilizing GO at 1.0 mg/ml on glass slides. Furthermore, we examined the osteogenic responses of murine mesenchymal-like stem cells, C3H10T1/2 cells, on GO substrates.
C3H10T1/2 cells on GO substrates resulted in increased cell surface area, enhanced cellular adhesions, and instigated osteogenic differentiation. Furthermore, priming of C3H10T1/2 cells with chondrocyte-conditioned medium (CM) could further induce a synergistic effect of osteogenesis on GO substrates.
All of these data suggest that GO substrate along with CM is suitable for upregulating osteogenic responses of mesenchymal stem cells.
组织工程是一个跨学科领域，试图通过仿生制造具有特定功能的支架来修复或再生组织和器官。近年来，氧化石墨烯（GO）因其无毒、高分散性和亲水相互作用而被认为是一种有前途的生物材料，这些特性是刺激底物与细胞之间相互作用的关键。
在本研究中，通过将1.0mg/ml的GO化学固定在载玻片上来制备GO底物。此外，我们检测了小鼠间充质样干细胞C3H10T1/2细胞在GO底物上的成骨反应。
C3H10T1/2细胞在GO底物上导致细胞表面积增加、细胞粘附增强，并促进成骨分化。此外，用软骨细胞条件培养基（CM）预处理C3H10T1/2细胞可进一步诱导GO底物上的成骨协同效应。
所有这些数据表明，GO底物与CM一起适用于上调间充质干细胞的成骨反应。
