Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Environ Sci Pollut Res Int. 2019 May;26(15):14712-14716. doi: 10.1007/s11356-018-2429-9. Epub 2018 Jun 5.
Tinospora crispa stem aqueous extractions for various time durations were determined regarding their total phenolic content and their larvicidal abilities. The results revealed that the total phenolic content in 1-, 3-, 5-, 10-, and 24-h extracts were 8.26, 8.43, 13.57, 12.52, and 12.43 mg/g gallic acid equivalent, respectively. The 5-h extract of T. crispa was evaluated against Culex quinquefasciatus mosquito larva in concentrations 3.125, 6.25, 12.5, and 25 mg/l, by determining the lethal concentration (LC) within 24 h and by histopathological analysis. The 24-h LC and LC values were 16.95 and 30.12 mg/l, respectively. The histopathological lesions after exposure to 50% of the 24-h LC were observed primarily in the midgut of the larva. The lesions observed were for the example epithelial cells lifting from the basement membrane, cell elongation protruding into the lumen, brush border disrupting with absent microvilli, and vesicle appearance. The present study indicated that the aqueous extract of this herb may have a suitable property for a larvicidal natural product and may replace harmful chemical pesticides.
不同时间提取的三叶崖爬藤茎水提物的总酚含量和杀幼虫活性。结果表明，1、3、5、10 和 24 h 提取物的总酚含量分别为 8.26、8.43、13.57、12.52 和 12.43 mg/g 没食子酸当量。5 h 提取物在 3.125、6.25、12.5 和 25 mg/l 浓度下用于防治致倦库蚊幼虫，在 24 h 内测定致死浓度（LC），并进行组织病理学分析。24 h LC 和 LC 值分别为 16.95 和 30.12 mg/l。暴露于 24 h LC 的 50%后，幼虫中肠主要出现组织病理学损伤。观察到的病变例如上皮细胞从基膜上抬起、细胞伸长突出到管腔、刷状缘破坏且微绒毛缺失以及出现囊泡。本研究表明，该植物的水提物可能具有作为一种杀幼虫天然产物的合适特性，并可能替代有害的化学农药。
