单纯疱疹病毒1型的立即早期增强子元件可替代c-Ha-ras1癌基因转化所需的调控区域。
The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation.
作者信息
Puga A, Gomez-Marquez J, Brayton P R, Cantin E M, Long L K, Barbacid M, Notkins A L
出版信息
J Virol. 1985 Jun;54(3):879-81. doi: 10.1128/JVI.54.3.879-881.1985.
Abstract
A 0.8-kilobase SacI DNA fragment in the distal 5'-noncoding region of the c-Ha-ras1 oncogene hybridized to high guanine X cytosine sites of herpes simplex virus type 1 (HSV-1) DNA restriction fragments. Nucleotide sequence comparisons localized one of these sites to the intergenic region of HSV between the immediate-early genes coding for IEmRNA-3 and IEmRNA-4/5 that has enhancer-type activity. We tested the possibility that the HSV-1 enhancer and the upstream c-Ha-ras1 SacI fragment were functionally related by assaying for the capacity of recombinant plasmids in which the HSV-1 enhancer replaced the oncogene 0.8-kilobase SacI fragment to transform NIH/3T3 cells. Deletion of the 0.8-kilobase SacI fragment abolished the biological activity of c-Ha-ras1, but its replacement by the HSV-1 enhancer fully restored it. These results confirm the enhancer properties of the HSV-1 immediate-early intergenic region and suggest that c-Ha-ras1 sequences contained within the 0.8-kilobase SacI fragment plays a role in the transcriptional activation of the oncogene.
摘要
c-Ha-ras1癌基因5'-非编码区远端的一个0.8千碱基的SacI DNA片段与单纯疱疹病毒1型（HSV-1）DNA限制片段的高鸟嘌呤X胞嘧啶位点杂交。核苷酸序列比较将其中一个位点定位到HSV编码IEmRNA-3和IEmRNA-4/5的立即早期基因之间的基因间区域，该区域具有增强子样活性。我们通过检测重组质粒（其中HSV-1增强子取代癌基因0.8千碱基的SacI片段）转化NIH/3T3细胞的能力，来测试HSV-1增强子与上游c-Ha-ras1 SacI片段在功能上是否相关。删除0.8千碱基的SacI片段消除了c-Ha-ras1的生物学活性，但其被HSV-1增强子取代后完全恢复了活性。这些结果证实了HSV-1立即早期基因间区域的增强子特性，并表明0.8千碱基SacI片段中包含的c-Ha-ras1序列在癌基因的转录激活中起作用。
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本文引用的文献
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A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.一个点突变导致了T24人膀胱癌癌基因获得转化特性。
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Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells.人类肿瘤细胞系中的癌基因：从人膀胱癌细胞中克隆一个转化基因
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