Samarut J, Mathey-Prevot B, Hanafusa H
Mol Cell Biol. 1985 May;5(5):1067-72. doi: 10.1128/mcb.5.5.1067-1072.1985.
We have studied the expression of the protein kinase activity of NCP98, the c-fps gene product, in several hemopoietic tissues of chickens as a function of the developmental stage of these organs. We found that in bone marrow, spleen, and bursa, maximum NCP98 kinase activity on a per-cell basis correlates with the peak of granulopoiesis in these organs. Furthermore, in a bovine serum albumin density gradient fractionation of bone marrow cells, granulocytic cells appeared to account for most of the NCP98 kinase activity. No correlation was found between the distribution of erythrocytic, lymphocytic, or thrombocytic cells and the distribution of the expression of NCP98 kinase activity. However, NCP98 protein and kinase activity were 10-fold higher in macrophages than in bone marrow. In addition, depletion by complement-mediated lysis of erythrocytic cells in bone marrow did not significantly reduce the total recovery of NCP98 kinase activity. These results argue for the specific expression of the c-fps gene product in granulocytic cells and macrophages.
我们研究了鸡的几种造血组织中NCP98（即c-fps基因产物）的蛋白激酶活性表达与这些器官发育阶段的关系。我们发现，在骨髓、脾脏和法氏囊中，基于细胞的NCP98激酶活性最大值与这些器官中粒细胞生成的峰值相关。此外，在骨髓细胞的牛血清白蛋白密度梯度分馏中，粒细胞似乎占了大部分NCP98激酶活性。红细胞、淋巴细胞或血小板细胞的分布与NCP98激酶活性表达的分布之间未发现相关性。然而，巨噬细胞中的NCP98蛋白和激酶活性比骨髓中的高10倍。此外，通过补体介导的骨髓红细胞裂解进行的消耗并没有显著降低NCP98激酶活性的总回收率。这些结果支持了c-fps基因产物在粒细胞和巨噬细胞中的特异性表达。
