Vizard D L, Yarsa J
Nucleic Acids Res. 1985 Jan 25;13(2):473-84. doi: 10.1093/nar/13.2.473.
The 393bp nucleotide sequence of a HindIII genomic fragment mapping within the major long interspersed repeated sequence family (MIF-1, Bam, L1) of mouse is reported and compared to clone sequences of the same region of this repeated sequence. The consensus of the clone sequences significantly differs from the genomic fragment sequence by additions and deletions that are inconsistent with the physical and biochemical properties of the genomic fragment. While alternative explanations could account for some of these differences, several aspects of the experimental results imply that cloning artifacts contribute to the discrepancies. Despite the differences between the clone and genomic fragment sequences, the biologically interesting features previously noted in clone sequences (promoter-like signals and an open reading frame) are conserved in the genomic fragment sequence.
报道了位于小鼠主要长散在重复序列家族（MIF - 1、Bam、L1）内的一个HindIII基因组片段的393bp核苷酸序列，并将其与该重复序列相同区域的克隆序列进行了比较。克隆序列的共有序列与基因组片段序列存在显著差异，这些差异表现为插入和缺失，且与基因组片段的物理和生化特性不一致。虽然其他解释可能说明其中一些差异，但实验结果的几个方面表明克隆假象导致了这些差异。尽管克隆序列和基因组片段序列存在差异，但先前在克隆序列中发现的生物学上有趣的特征（启动子样信号和一个开放阅读框）在基因组片段序列中得以保留。
