Norström A, Wiqvist I
Acta Endocrinol (Copenh). 1985 May;109(1):122-5. doi: 10.1530/acta.0.1090122.
The effects of porcine relaxin on the levels of cAMP in human cervical tissue were studied in vitro. The specimens were obtained by needle biopsy from women undergoing hysterectomy, legal abortion in the first trimester or elective Caesarean section at term, and were incubated in Krebs-Ringer buffer for 15 min in the presence of porcine relaxin (5 micrograms/ml, 3000 GPU/mg). cAMP was determined using a modified protein binding assay. The concentration of cAMP was higher in pregnant than in non-pregnant women. Relaxin stimulated the production of cAMP in the 7th-8th week of gestation and at term but did not significantly alter the cervical cAMP levels in neither non-pregnant women nor in women in the 10th-12th week of pregnancy. Previous studies have shown that porcine relaxin reduces collagen synthesis in tissue from the human cervix and lower uterine segment. The present observations indicate that these effects can be mediated by cAMP.
在体外研究了猪松弛素对人宫颈组织中环磷酸腺苷（cAMP）水平的影响。标本通过针吸活检从接受子宫切除术、孕早期合法堕胎或足月选择性剖宫产的女性中获取，并在含有猪松弛素（5微克/毫升，3000国际单位/毫克）的Krebs-Ringer缓冲液中孵育15分钟。使用改良的蛋白结合测定法测定cAMP。孕妇体内cAMP的浓度高于非孕妇。松弛素在妊娠第7 - 8周和足月时刺激cAMP的产生，但在非孕妇或妊娠第10 - 12周的女性中均未显著改变宫颈cAMP水平。先前的研究表明，猪松弛素可减少人宫颈和子宫下段组织中的胶原蛋白合成。目前的观察结果表明，这些作用可能由cAMP介导。
