Bolin C A, Bolin S R, Kluge J P, Mengeling W L
Am J Vet Res. 1985 May;46(5):1039-42.
Pseudorabies virus was inoculated into the uterus of 15 gilts within 6 hours after natural breeding, and gilts were necropsied at postbreeding days (PBD) 3, 6, 10, 14, and 28; 3 control gilts were treated similarly, except for inoculation with pseudorabies virus and were necropsied at PBD 6, 10, and 14. Tissues were collected for virus isolation, fluorescent antibody staining, and histopathologic examination. Pseudorabies virus was isolated from the reproductive tract up to day 14. Lesions in the reproductive tract consisted of multifocal to diffuse lymphohistiocytic vaginitis and endometritis, and lymphoplasmacytic aggregates in the corpora lutea. Multiple ulcers were seen in the vagina or endometrium of several gilts at PBD 3, 6, and 10. Corpora lutea of 1 gilt were necrotic at PBD 14 and contained large numbers of inflammatory cells. Focal aggregates of lymphocytes and plasma cells were seen in vagina and endometrium of 3 gilts and in the ovary of 1 gilt at day 28.
在自然配种后6小时内，将伪狂犬病病毒接种到15头后备母猪的子宫内，并在配种后天数（PBD）3、6、10、14和28对后备母猪进行剖检；3头对照后备母猪接受类似处理，但未接种伪狂犬病病毒，并在PBD 6、10和14进行剖检。收集组织用于病毒分离、荧光抗体染色和组织病理学检查。直到第14天，均从生殖道中分离出伪狂犬病病毒。生殖道病变包括多灶性至弥漫性淋巴组织细胞性阴道炎和子宫内膜炎，以及黄体中的淋巴浆细胞聚集。在PBD 3、6和10时，几头后备母猪的阴道或子宫内膜出现多处溃疡。1头后备母猪的黄体在PBD 14时坏死，且含有大量炎性细胞。在第28天，3头后备母猪的阴道和子宫内膜以及1头后备母猪的卵巢中可见淋巴细胞和浆细胞的局灶性聚集。
