Infectious Diseases and Tropical Medicine Research Center, Department of Medical Parasitology and Mycology, Babol University of Medical Sciences, Babol, Iran.
Department of Prosthodontics, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.
Biomed Res Int. 2018 May 16;2018:3086586. doi: 10.1155/2018/3086586. eCollection 2018.
-associated denture stomatitis (CADS) is a common fungal infection in people who wear dentures. The main objective of this study was to make molecular identification of causative agents of CADS and in vitro antifungal susceptibility testing (AFST) in the Iranian patients with denture stomatitis.
A total of 134 spp. were obtained from patients with denture stomatitis. The spp. were identified using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) involving the universal internal transcribed spacer (ITS1 and ITS4) primers, which were subjected to digestion with MspI and BlnI restriction enzymes. The in vitro antifungal susceptibility of spp. to fluconazole (FLC), terbinafine (TRB), itraconazole (ITC), voriconazole (VRC), posaconazole (POS), ketoconazole (KET), amphotericin B (AMB), and caspofungin (CAS) was evaluated using the Clinical and Laboratory Standards Institute M27-A3 and M27-S4 guidelines.
Overall, was the most commonly isolated species ( = 84; 62.6%), followed by ( = 23; 17.2%), ( = 16; 12%), and ( = 11; 8.2%). Posaconazole had the lowest geometric mean minimum inhibitory concentration (MIC) (0.03 g/ml), followed by AMB (0.05 g/ml), ITC (0.08 g/ml), VRC (0.11 g/ml), CAS (0.12 g/ml), KET (0.15 g/ml), and FLC (0.26 g/ml).
Our study showed that was most prevalent in Iranian patients with CADS and was susceptible to both azoles and amphotericin B. In addition, POS could be an appropriate alternative to the current antifungal agents used for the treatment of CADS, as well as in the treatment of recurrent candidiasis.
-相关义齿性口炎（CADS）是佩戴义齿人群中常见的真菌感染。本研究的主要目的是对伊朗义齿性口炎患者的致病因子进行分子鉴定和体外抗真菌药敏试验（AFST）。
从义齿性口炎患者中获得了共 134 株 spp.。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP），涉及通用内部转录间隔区（ITS1 和 ITS4）引物，对 spp.进行鉴定，然后用 MspI 和 BlnI 限制性内切酶进行消化。根据临床和实验室标准协会 M27-A3 和 M27-S4 指南，评估 spp.对氟康唑（FLC）、特比萘芬（TRB）、伊曲康唑（ITC）、伏立康唑（VRC）、泊沙康唑（POS）、酮康唑（KET）、两性霉素 B（AMB）和卡泊芬净（CAS）的体外抗真菌敏感性。
总体而言， 是最常分离的物种（=84；62.6%），其次是 （=23；17.2%）、 （=16；12%）和 （=11；8.2%）。泊沙康唑具有最低的几何平均最小抑菌浓度（MIC）（0.03 g/ml），其次是两性霉素 B（0.05 g/ml）、伊曲康唑（0.08 g/ml）、伏立康唑（0.11 g/ml）、卡泊芬净（0.12 g/ml）、酮康唑（0.15 g/ml）和氟康唑（0.26 g/ml）。
本研究表明， 在伊朗 CADS 患者中最为普遍，对唑类和两性霉素 B 均敏感。此外，POS 可能是治疗 CADS 和复发性念珠菌病的现有抗真菌药物的替代药物。
