Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia.
Carbohydr Polym. 2018 Sep 15;196:279-288. doi: 10.1016/j.carbpol.2018.05.045. Epub 2018 May 21.
New composites based on Ca-alginate hydrogels were produced that release activated charcoal (AC) particles with adsorbed povidone iodine (PVP-I) as a model antimicrobial substance in a physiological-like environment. Composite beads with different alginate (0.5-1.5%w/w) and AC (1-20%w/w) concentrations were analyzed by FE-SEM and characterized regarding textural parameters, swelling, and AC release kinetics. PVP-I was easily adsorbed onto AC particles within the optimized beads (0.5%w/w alginate, 20%w/w AC) as indicated by UV-vis spectroscopy, EDX and FT-IR analyses. The obtained beads have shown strong bactericidal effects against two standard bacterial strains (Staphylococcus aureus and Pseudomonas aeruginosa) and clinical multi-resistant wound isolates (MRSA, Escherichia coli, Pseudomonas aeruginosa, Еnterococcus faecalis and Proteus mirabilis) and, at the same time, exhibited negligible PVP-I desorption in physiological saline solution. Thus, the obtained composites could provide utilization of potent antiseptics such as iodine, in wound dressings, without the concern of systemic absorption.
制备了基于海藻酸钠水凝胶的新型复合材料，该复合材料在类似生理的环境中释放载有吸附聚维酮碘（PVP-I）的活性炭（AC）颗粒作为模型抗菌物质。通过 FE-SEM 分析和对结构参数、溶胀和 AC 释放动力学进行了表征，研究了不同海藻酸钠（0.5-1.5%w/w）和 AC（1-20%w/w）浓度的复合珠。如 UV-vis 光谱、EDX 和 FT-IR 分析所示，PVP-I 很容易被吸附到优化后的珠粒（0.5%w/w 海藻酸钠、20%w/w AC）中的 AC 颗粒上。所得到的珠粒对两种标准细菌菌株（金黄色葡萄球菌和铜绿假单胞菌）和临床多耐药性伤口分离株（MRSA、大肠杆菌、铜绿假单胞菌、粪肠球菌和奇异变形杆菌）表现出强烈的杀菌作用，同时在生理盐水中表现出可忽略不计的 PVP-I 解吸。因此，所得到的复合材料可在不担心全身吸收的情况下，在伤口敷料中利用碘等有效的防腐剂。
