Bankier A T, Dietrich W, Baer R, Barrell B G, Colbère-Garapin F, Fleckenstein B, Bodemer W
J Virol. 1985 Jul;55(1):133-9. doi: 10.1128/JVI.55.1.133-139.1985.
The H-DNA repeat unit of Herpesvirus saimiri strain 11 was cloned in plasmid vector pAGO, and the nucleotide sequence was determined by the dideoxy chain termination method. One unit of repetitive DNA has 1,444 base pairs with 70.8% G+C content. The structural features of repeat DNA sequences at the termini of intact virion M-DNA (160 kilobases) and orientation of reiterated DNA were analyzed by radioactive end labeling of M-DNA, followed by cleavage of the end fragments with restriction endonucleases. The termini appeared to be blunt ended with a 5'-phosphate group, probably generated during encapsidation by cleavage in the immediate vicinity of the single ApaI recognition site in the H-DNA repeat unit. The sequence did not reveal sizeable open reading frames, the longest hypothetical peptide from H-DNA being 85 amino acids. There was no evidence for an mRNA promoter or terminator element, and H-DNA-specific transcription could not be found in productively infected cells.
将赛氏疱疹病毒11株的H-DNA重复单元克隆到质粒载体pAGO中，并用双脱氧链终止法测定核苷酸序列。一个重复DNA单元有1444个碱基对，G+C含量为70.8%。通过对M-DNA进行放射性末端标记，然后用限制性内切酶切割末端片段，分析了完整病毒粒子M-DNA（160千碱基）末端重复DNA序列的结构特征和重复DNA的方向。末端似乎是平端的，带有一个5'-磷酸基团，可能是在衣壳化过程中，在H-DNA重复单元中单一ApaI识别位点的紧邻区域进行切割而产生的。该序列未显示出可观的开放阅读框，来自H-DNA的最长假设肽为85个氨基酸。没有证据表明存在mRNA启动子或终止子元件，并且在有效感染的细胞中未发现H-DNA特异性转录。
