大肠杆菌rglA和rglB基因座的转座子诱变及基因定位
Transposon mutagenesis and genetic mapping of the rglA and rglB loci of Escherichia coli.
作者信息
Ravi R S, Sozhamannan S, Dharmalingam K
出版信息
Mol Gen Genet. 1985;198(3):390-2. doi: 10.1007/BF00332928.
PMID:2989655
Abstract
The rglA and rglB genes code for two different proteins which cleave the hydroxymethylated cytosine residues of T-even phages. We isolated Tn10 and Tn5 insertion mutants of the above genes and of the genes in and around the rglA and rglB loci. These insertions were used to construct a detailed genetic map. Our results show that the rglA gene maps at 25.24 min and the rglB gene at 98.39 min on the standard Escherichia coli K12 genetic map.
摘要
rglA和rglB基因编码两种不同的蛋白质，它们可切割T偶数噬菌体的羟甲基化胞嘧啶残基。我们分离出了上述基因以及rglA和rglB基因座内及其周围基因的Tn10和Tn5插入突变体。这些插入用于构建详细的遗传图谱。我们的结果表明，在标准大肠杆菌K12遗传图谱上，rglA基因位于25.24分钟处，rglB基因位于98.39分钟处。
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