Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece.
Section of Pharmaceutical Technology, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens 15771, Greece.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):69-75. doi: 10.1016/j.ijbiomac.2018.06.050. Epub 2018 Jun 15.
Ulvan, a marine sulfated polysaccharide isolated from green algae, has been recently recognized as a natural biopolymer of biomedical interest. A series of lysozyme/ulvan complexes prepared under various charge ratios at physiological pH were studied. The resulting complexes were examined with light scattering techniques in order to characterize the size, the distribution and the ζ-potential of the nanocarriers, which were found to depend on the charge ratio employed. Increased complexation efficiency of lysozyme was observed for certain charge ratios, while ATR-FTIR data suggested that the protein structure after complexation was retained. Bacterial growth studies showed that lysozyme once complexed with ulvan not only retains its antibacterial activity against the Gram positive strain Staphylococcus aureus, but actually exhibits increased levels of activity. In this model study, the results highlight the potential of ulvan as a promising nanocarrier for positively charged bioactive molecules.
岩藻聚糖，一种从绿藻中分离出的海洋硫酸多糖，最近被认为是一种具有生物医学意义的天然生物聚合物。在生理 pH 值下，以不同的电荷比制备了一系列溶菌酶/岩藻聚糖复合物，并对其进行了研究。使用光散射技术对所得复合物进行了检查，以表征纳米载体的大小、分布和 ζ 电位，结果发现这些参数取决于所使用的电荷比。在某些电荷比下，观察到溶菌酶的复合物化效率增加，而 ATR-FTIR 数据表明复合物化后蛋白质结构得以保留。细菌生长研究表明，溶菌酶一旦与岩藻聚糖复合，不仅保留了其对革兰氏阳性菌金黄色葡萄球菌的抗菌活性，而且实际上还表现出更高的活性。在这项模型研究中，结果强调了岩藻聚糖作为带正电荷的生物活性分子的有前途的纳米载体的潜力。
