Dürst M, Kleinheinz A, Hotz M, Gissmann L
J Gen Virol. 1985 Jul;66 ( Pt 7):1515-22. doi: 10.1099/0022-1317-66-7-1515.
Cloned DNA from human papillomavirus (HPV) type 16 was subjected to restriction enzyme analysis. A genome size of 7.8 +/- 0.1 kb was determined and restriction maps were prepared. Fragments of HPV 16 DNA were nick-translated and hybridized with fragments of HPV 6b DNA. The two genomes appeared to be colinear. The physical state of HPV 16 DNA in genital tumours was analysed. In each of six benign tumours the viral DNA was detected exclusively as 8 kb circles. In four malignant tumours the viral DNA appeared to be integrated within the host genome but one cervical carcinoma and one case of Bowen's disease also contained oligomeric episomal molecules of viral DNA. One cervical carcinoma (WV 2965), containing only integrated viral DNA, was examined in detail. HPV 16 DNA was integrated as head-to-tail tandem repeats at more than one site. Three virus/cell junction fragments from this tumour were cloned. Two contained lengths of repetitive cellular DNA and one a length of apparently single copy cellular DNA.
对来自16型人乳头瘤病毒（HPV）的克隆DNA进行了限制性内切酶分析。确定了基因组大小为7.8±0.1 kb，并绘制了限制性图谱。对HPV 16 DNA片段进行缺口平移，然后与HPV 6b DNA片段杂交。这两个基因组似乎是共线的。分析了生殖器肿瘤中HPV 16 DNA的物理状态。在六个良性肿瘤中的每一个中，病毒DNA仅作为8 kb的环状物被检测到。在四个恶性肿瘤中，病毒DNA似乎整合到宿主基因组中，但一例宫颈癌和一例鲍恩病也含有病毒DNA的寡聚游离型分子。对仅含有整合病毒DNA的一例宫颈癌（WV 2965）进行了详细检查。HPV 16 DNA以头对头串联重复的形式整合在多个位点。克隆了来自该肿瘤的三个病毒/细胞连接片段。两个片段含有一段重复的细胞DNA，一个片段含有一段明显的单拷贝细胞DNA。
