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Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase gene.

作者信息

Rodriguez J F, Kahn J S, Esteban M

出版信息

Proc Natl Acad Sci U S A. 1986 Dec;83(24):9566-70. doi: 10.1073/pnas.83.24.9566.

DOI:10.1073/pnas.83.24.9566
PMID:3025846
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC387181/
Abstract

A rabbit poxvirus genomic library contained within the expression vector lambda gt11 was screened with polyclonal antiserum prepared against vaccinia virus nucleic acid-dependent nucleoside triphosphatase (NTPase)-I enzyme. Five positive phage clones containing from 0.72- to 2.5-kilobase-pair (kbp) inserts expressed a beta-galactosidase fusion protein that was reactive by immunoblotting with the NTPase-I antibody. Hybridization analysis allowed the location of this gene within the vaccinia HindIIID restriction fragment. From the known nucleotide sequence of the 16-kbp vaccinia HindIIID fragment, we identified a region that contains a 1896-base open reading frame coding for a 631-amino acid protein. Analysis of the complete sequence revealed a highly basic protein, with hydrophilic COOH and NH2 termini, various hydrophobic domains, and no significant homology to other known proteins. Translational studies demonstrate that NTPase-I belongs to a late class of viral genes. This protein is highly conserved among Orthopoxviruses.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/d4610501f2d2/pnas00328-0303-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/6adf9154df72/pnas00328-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/26f21d49c740/pnas00328-0302-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/834a749e461c/pnas00328-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/24c1568f7b54/pnas00328-0303-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/3d3f863f8e90/pnas00328-0303-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/0913d1b555cd/pnas00328-0303-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/5d959ecea1ae/pnas00328-0303-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/29465ca32ee1/pnas00328-0303-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/d4610501f2d2/pnas00328-0303-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/6adf9154df72/pnas00328-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/26f21d49c740/pnas00328-0302-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/834a749e461c/pnas00328-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/24c1568f7b54/pnas00328-0303-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/3d3f863f8e90/pnas00328-0303-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/0913d1b555cd/pnas00328-0303-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/5d959ecea1ae/pnas00328-0303-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/29465ca32ee1/pnas00328-0303-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5006/387181/d4610501f2d2/pnas00328-0303-g.jpg

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Determinants of vaccinia virus early gene transcription termination.

本文引用的文献

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Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus gene.痘苗病毒早期基因多个起始和终止位点附近的独特核苷酸序列。
Cell. 1981 Sep;25(3):805-13. doi: 10.1016/0092-8674(81)90188-4.
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Complete nucleotide sequences of two adjacent early vaccinia virus genes located within the inverted terminal repetition.位于反向末端重复序列内的两个相邻痘苗病毒早期基因的完整核苷酸序列。
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A simple method for displaying the hydropathic character of a protein.
痘苗病毒早期基因转录终止的决定因素。
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Analysis of the monkeypox virus genome.猴痘病毒基因组分析。
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Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase of the DExH box family.痘苗病毒核苷三磷酸磷酸水解酶I（一种DExH盒家族的DNA依赖性ATP酶）的突变分析
J Virol. 1999 Feb;73(2):1302-8. doi: 10.1128/JVI.73.2.1302-1308.1999.
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Three adjacent genes of African swine fever virus with similarity to essential poxvirus genes.非洲猪瘟病毒的三个相邻基因，与痘病毒必需基因具有相似性。
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Vaccinia virus nucleoside triphosphate phosphohydrolase I controls early and late gene expression by regulating the rate of transcription.痘苗病毒核苷三磷酸磷酸水解酶I通过调节转录速率来控制早期和晚期基因表达。
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Targeting of a multicomponent transcription apparatus into assembling vaccinia virus particles requires RAP94, an RNA polymerase-associated protein.将多组分转录装置靶向装配中的痘苗病毒颗粒需要RAP94，一种与RNA聚合酶相关的蛋白质。
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Ann N Y Acad Sci. 1982;402:45-64. doi: 10.1111/j.1749-6632.1982.tb25731.x.