Naz Sadia, Umair Muhammad, Iqbal Sajid
Animal Reproduction & Genetics Program, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan.
Semen Production Unit Qadirabad, Sahiwal, Pakistan.
Andrologia. 2018 Oct;50(8):e13063. doi: 10.1111/and.13063. Epub 2018 Jun 19.
Our objectives were to ascertain the comparison of Tris egg yolk-based, Triladyl and Optixell extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n = 35) from five bulls were diluted in Tris egg yolk-based, Triladyl , Optixell extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher (p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk-based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross-frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher (p < 0.05) in spermatozoa cryopreserved in Optixell extender as compared to Tris egg yolk-based and Triladyl extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher (p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk-based and Triladyl extender respectively. It is concluded that Optixell extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.
我们的目标是确定基于Tris蛋黄的稀释液、Triladyl和Optixell稀释液对尼里-拉菲水牛（Bubalus bubalis）公牛精子解冻后质量、计算机辅助精子分析（CASA）参数和体内受精能力的影响。从五头公牛采集的精液样本（n = 35）用基于Tris蛋黄的稀释液、Triladyl、Optixell稀释液进行稀释，并装入0.50毫升的法式细管中冷冻。与Triladyl和基于Tris蛋黄的稀释液相比，Optixell稀释液解冻后精子的CASA活力（%）更高（p < 0.05）。虽然与基于Tris蛋黄的稀释液和Triladyl稀释液相比，用Optixell稀释液冷冻保存的精子的渐进性活力（%）、形态（%）、平均路径速度（μm/s）、直线速度（μm/s）、曲线速度（μm/s）、头部侧向位移幅度（μm）、鞭打交叉频率（Hz）、直线性（%）、曲线路径长度（μm）、平均路径长度（μm）、完整的质膜和顶体膜（%）以及DNA完整性（%）更高（p < 0.05）。与分别用基于Tris蛋黄的稀释液和Triladyl稀释液冷冻的精液剂量进行人工授精的水牛相比，用Optixell稀释液冷冻的精液剂量进行人工授精的水牛的受胎率（68.18%、45.45%、55.4%）更高（p < 0.05）。得出的结论是，Optixell稀释液可提高尼里-拉菲水牛解冻后精液的质量和受胎率。
