开发用于生成肺组织以进行个性化疾病建模的三维生物工程技术。
Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling.
作者信息
Wilkinson Dan C, Mellody Michael, Meneses Luisa K, Hope Ashley C, Dunn Bruce, Gomperts Brigitte N
机构信息
Department of Materials Science and Engineering, UCLA, Los Angeles, California.
Department of Bioengineering, UCLA, Los Angeles, California.
出版信息
Curr Protoc Stem Cell Biol. 2018 Aug;46(1):e56. doi: 10.1002/cpsc.56. Epub 2018 Jun 7.
Abstract
This unit describes a protocol for generation of lung organoids. A lung organoid is a 3D cell/hydrogel composite that resembles the morphology and cellular composition of the human distal lung. These tissue-engineered constructs provide an in vitro model of human lung and are best suited for disease modeling applications. The organoid generation methodology is flexible, allowing for easy scalability in the number of organoids produced and in the ability to accommodate a wide range of cell types. © 2018 by John Wiley & Sons, Inc.
摘要
本单元描述了一种生成肺类器官的方案。肺类器官是一种三维细胞/水凝胶复合物，其形态和细胞组成类似于人类远端肺。这些组织工程构建体提供了一种人类肺的体外模型，最适合用于疾病建模应用。类器官生成方法具有灵活性，能够轻松扩大所生产类器官的数量，并能容纳多种细胞类型。© 2018 约翰威立国际出版公司
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