Cebrián-García Soledad, Balu Alina M, Luque Rafael
Departamento de Quimica Organica, Universidad de Cordoba, Cordoba, Spain.
Scientific Center for Molecular Design and Synthesis of Innovative Compounds for the Medical Industry, Peoples Friendship University of Russia (RUDN), Moscow, Russia.
Front Chem. 2018 Jun 7;6:197. doi: 10.3389/fchem.2018.00197. eCollection 2018.
A novel, environmentally friendly, and sustainable ultrasound-assisted methodology in the valorization of valeric acid to alkyl valerate using a biosilicified lipase from is reported. This one-pot room temperature methodology of enzyme biosilicification leads to biosilicified lipases with improved activity and reaction efficiency as compared to free enzymes. Yields in the ultrasound-promoted esterification of valeric acid was ca. 90% in 2 h with 15% m/v of biosilicified lipase (Bio-lipase; 616 U/g enzymatic activity) and a molar ratio 1:2 (valeric acid:ethanol), slightly superior to that observed by the free enzyme (75% conversion, 583U/g enzymatic activity). The reuse of enzymes in these conditions was tested and the results show a relatively good reusability of these biosilicified enzymes under the investigated conditions, particularly preserving fairly stable specific activities (616 vs. 430 U/g after four reuses).
报道了一种新型、环保且可持续的超声辅助方法，该方法利用来自[具体来源未给出]的生物硅化脂肪酶将戊酸转化为戊酸烷基酯。这种酶生物硅化的一锅法室温方法所产生的生物硅化脂肪酶，与游离酶相比，具有更高的活性和反应效率。在戊酸的超声促进酯化反应中，使用15%（m/v）的生物硅化脂肪酶（生物脂肪酶；酶活性为616 U/g）和1:2的摩尔比（戊酸:乙醇），在2小时内产率约为90%，略高于游离酶所观察到的产率（转化率75%，酶活性583 U/g）。测试了这些条件下酶的重复使用情况，结果表明这些生物硅化酶在研究条件下具有相对良好的重复使用性，特别是在四次重复使用后仍能保持相当稳定的比活性（616 U/g对430 U/g）。
