Detection of BK virus antibodies measured by enzyme-linked immunosorbent assay (ELISA) and two haemagglutination inhibition methods: a comparative study.

We developed a solid phase enzyme-linked immunosorbent assay (ELISA) for detecting IgG antibodies to BK virus (BKV). This test was compared to two haemagglutination inhibition methods, one using receptor-destroying enzyme and the other NaIO4 to remove nonspecific inhibitors. The results show that this single dilution ELISA is a sensitive and specific test. The inherent advantages of this method make it an attractive alternative for the seroepidemiology of BKV.