Flaegstad T, Traavik T
J Med Virol. 1985 Oct;17(2):195-204. doi: 10.1002/jmv.1890170212.
We have used an antigen solid-phase enzyme-linked immunosorbent assay (SP-ELISA) and an IgM antibody capture ELISA (MACELISA) for detecting IgM antibodies to human polyomavirus BK (BKV). These tests were compared with the standard hemagglutination inhibition test (HAI) of IgM serum fractions following sucrose density gradient fractionation. The SP- and MACELISA were not influenced by concomitant BKV-IgG, but high levels of both BKV-IgG and rheumatoid factor could cause false positive results by SPELISA, but not by MACELISA. The MACELISA gave much higher positive to negative ratios than the SPELISA. The sensitivity and specificity of the two tests were high compared to the IgM-HAI method. The sera could be tested in a single dilution (1:160), and thus the ELISA-tests are useful for testing large numbers of sera.
我们使用了抗原固相酶联免疫吸附测定法（SP - ELISA）和IgM抗体捕获ELISA法（MACELISA）来检测人多瘤病毒BK（BKV）的IgM抗体。将这些检测方法与蔗糖密度梯度分级分离后IgM血清组分的标准血凝抑制试验（HAI）进行了比较。SP - ELISA和MACELISA不受同时存在的BKV - IgG的影响，但高水平的BKV - IgG和类风湿因子均可导致SP - ELISA出现假阳性结果，而MACELISA则不会。MACELISA的阳性与阴性比值远高于SP - ELISA。与IgM - HAI方法相比，这两种检测方法的敏感性和特异性都很高。血清可以用单一稀释度（1:160）进行检测，因此ELISA检测方法对于检测大量血清很有用。
