Flaegstad T, Traavik T, Christie K E, Joergensen J
J Med Virol. 1986 Jul;19(3):287-96. doi: 10.1002/jmv.1890190311.
We developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzyme-linked immunosorbent assay (ELISA) were able to prevent expression of BKV structural antigens in cell cultures. The correlation between titers in the hemagglutination inhibition test, levels of BKV IgG measured by ELISA, and the titers assayed by the immunoperoxidase neutralization test was high. We suggest that this type of test may be used instead of conventional neutralization tests for other viruses with slowly developing cytopathogenic effects.
我们开发了一种免疫过氧化物酶染色试验，用于检测Vero细胞培养物中BK病毒（BKV）的结构抗原。该试验用于检测人和免疫动物血清的中和活性。结果表明，通过血凝抑制试验和酶联免疫吸附测定（ELISA）检测为BKV抗体阳性的血清能够阻止BKV结构抗原在细胞培养物中的表达。血凝抑制试验中的滴度、ELISA检测的BKV IgG水平与免疫过氧化物酶中和试验检测的滴度之间具有高度相关性。我们建议，对于其他具有缓慢发展的细胞病变效应的病毒，这种类型的试验可替代传统的中和试验。
