Flaegstad T, Traavik T, Kristiansen B E
J Hyg (Lond). 1986 Jun;96(3):523-8. doi: 10.1017/s0022172400066328.
Enzyme immunoassays (ELISA) have been developed for the detection of BK virus IgG- and IgM-antibodies. Specific IgG is detected by an antigen-coated solid phase test; IgM by an antibody capture method. These methods have been used to study the age-distribution of BK virus antibodies in Tromsø county in Northern Norway. The serum panels tested were: 60 sera from paediatric patients aged 0-1 year; 220 sera from healthy persons aged 1-82 years; 74 sera from healthy blood donors; 107 sera from healthy pregnant women. The age-distribution of BKV-IgG antibodies showed that primary infections took place predominantly between the ages of 1 and 6 years, and that there were no sex differences, either in the age-specific prevalence or in the level of BKV-IgG. We found no significant differences in the prevalence of BKV-IgM antibodies in healthy children and adults and pregnant women. BKV-IgM was detected in 26 of the 461 sera tested (5.6%).
已经开发出酶免疫测定法（ELISA）用于检测BK病毒IgG和IgM抗体。通过抗原包被的固相试验检测特异性IgG；通过抗体捕获法检测IgM。这些方法已用于研究挪威北部特罗姆瑟郡BK病毒抗体的年龄分布。所检测的血清样本包括：60份来自0至1岁儿科患者的血清；220份来自1至82岁健康人的血清；74份来自健康献血者的血清；107份来自健康孕妇的血清。BKV-IgG抗体的年龄分布表明，初次感染主要发生在1至6岁之间，并且在年龄特异性患病率或BKV-IgG水平方面没有性别差异。我们发现健康儿童、成人和孕妇中BKV-IgM抗体的患病率没有显著差异。在所检测的461份血清中有26份（5.6%）检测到BKV-IgM。
