Flaegstad T, Traavik T, Kolmannskog S, Stokland T
Virological Research Group, University of Tromsø, Norway.
J Med Virol. 1988 Jan;24(1):33-44. doi: 10.1002/jmv.1890240106.
We recently developed enzyme-linked immunosorbent assays (ELISAs) for the detection of anti-BK virus IgG- and IgM-antibodies, and also a convenient and rapid serum neutralization test. Complemented by a traditional haemagglutination inhibition test (HAI) these methods were used to investigate the longitudinal response to BKV infection in sequentially taken sera from 29 children under treatment for cancer. In separate experiments it was shown that the results were not at any extent influenced by antibodies against other polyomaviruses. At the time of diagnosis the prevalence of specific IgG- and IgM-antibodies and the geometric mean IgG levels were not significantly different for the patients compared with a group of healthy children. The primary infections seemed to occur at the same age for the two groups of children. Seven of the patients had a primary infection with BKV. The results indicate that the host response in moderately immunosuppressed children during primary infection is the same as expected for healthy individuals with the development of specific IgG, HAI, and NT antibodies, and, usually, production of BKV-IgM for several months. The results indicated that whether specific IgM was demonstrated in the first sample or appeared later during a reactivation episode, this parameter was correlated with profound immunosuppression. Significant titre changes, detectable IgM antibodies and/or seroconversions were demonstrated in 69% (20/29) of the cancer patients. Such indications of recent viral activity was recorded in 42% (8/19) of children with meningococcal infections. The observation periods for the two groups of patients are, however, not directly comparable.
我们最近开发了用于检测抗BK病毒IgG和IgM抗体的酶联免疫吸附测定（ELISA），以及一种便捷快速的血清中和试验。辅以传统的血凝抑制试验（HAI），这些方法被用于研究29名接受癌症治疗的儿童连续采集的血清中对BKV感染的纵向反应。在单独的实验中表明，结果在任何程度上都不受针对其他多瘤病毒抗体的影响。在诊断时，与一组健康儿童相比，患者中特异性IgG和IgM抗体的患病率以及几何平均IgG水平没有显著差异。两组儿童的初次感染似乎发生在相同年龄。其中7名患者初次感染了BKV。结果表明，中度免疫抑制儿童在初次感染期间的宿主反应与健康个体预期的相同，即产生特异性IgG、HAI和中和抗体（NT），并且通常在几个月内产生BKV-IgM。结果表明，无论特异性IgM是在第一个样本中检测到还是在再激活发作期间稍后出现，该参数都与深度免疫抑制相关。69%（20/29）的癌症患者出现了显著的滴度变化、可检测到的IgM抗体和/或血清转化。在42%（8/19）的脑膜炎球菌感染儿童中记录到了近期病毒活动的此类迹象。然而，两组患者的观察期没有直接可比性。
