Kherd Ali A, Helmi Nawal, Balamash Khadijah Saeed, Kumosani Taha A, Al-Ghamdi Shareefa A, Qari M, Huwait Etimad A, Yaghmoor Soonham S, Nabil Alaama, Al-Ghamdi Maryam A, Moselhy Said S
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Experimental Biochemistry Unit, King Fahd Medical Research center KFMRC, Jeddah, Kingdom of Saudi Arabia.
Afr Health Sci. 2017 Dec;17(4):1204-1210. doi: 10.4314/ahs.v17i4.31.
Studies have shown that Na-K ATPase activity was altered in disrupted red blood cell membranes and this enzyme is believed to be the site of active transport of Na and K in intact red blood cells. The enzyme is often referred to as Na-K pump because it pumps Na out and K into the cell against gradients with the concomitant hydrolysis of intracellular ATP.
The aim of this study was to find out the possibility of using Na-K-ATPase activity as a biomarker for the diagnosis of individuals with different physiological conditions.
The activity of Na-K ATPase was determined in blood samples collected from different pathological and physiological conditions such as pregnancy, smoking, diabetes and renal dysfunction compared with healthy subjects matched for age and sex.
The Na-K ATPase activity in pregnancy (0.094 ± 0.0051 µM Pi/min. mg protein), smoking (0.064 ± 0.0011 µM), diabetes (0.047 µM 0.002 µM) and kidney disease (0.069 ± 0.0014 µM) was higher compared to the measurements in healthy individuals (0.0081 ± 0.0031 µM).
Na-KATPase specific activity is a biomarker for the diagnosis of individuals with different physiological diseases.
研究表明，在破损的红细胞膜中钠钾ATP酶活性会发生改变，并且该酶被认为是完整红细胞中钠和钾主动转运的位点。这种酶常被称为钠钾泵，因为它逆浓度梯度将钠泵出细胞，同时将钾泵入细胞，并伴随着细胞内ATP的水解。
本研究的目的是探究将钠钾ATP酶活性用作诊断不同生理状况个体的生物标志物的可能性。
测定从不同病理和生理状况（如妊娠、吸烟、糖尿病和肾功能不全）采集的血样中钠钾ATP酶的活性，并与年龄和性别匹配的健康受试者进行比较。
与健康个体（0.0081±0.0031微摩尔）的测量值相比，妊娠（0.094±0.0051微摩尔无机磷/分钟·毫克蛋白质）、吸烟（0.064±0.0011微摩尔）、糖尿病（0.047微摩尔±0.002微摩尔）和肾脏疾病（0.069±0.0014微摩尔）中的钠钾ATP酶活性更高。
钠钾ATP酶比活性是诊断不同生理疾病个体的生物标志物。
