Ajiboye B O, Ekundayo B E, Salami A W, Osukoya A O, Komolafe K, Gaur S, Oyinloye B E, Jeje T O, Ojo O A
Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado, Ekiti, Nigeria.
Toxicol Rep. 2024 Sep 18;13:101742. doi: 10.1016/j.toxrep.2024.101742. eCollection 2024 Dec.
Studies suggest that medicinal plant extracts can help reduce the neuron degeneration associated with diabetes. In this study, the neuroprotective effect of the alkaloid-rich extract from the leaves of was assessed in rats with diabetes induced by streptozotocin (STZ).
alkaloid-rich analysis was carried out via a known procedure. The rats were randomly assigned into five treatment groups (n = 8): normal control, diabetic-induced rats (45 mg/kg STZ), and diabetic rats treated with low doses of leaf alkaloid-rich extract (50 mg/kg b.w, LEL) and high (100 mg/kg b.w, LEH) (300 mg/kg and 150 mg/kg), and metformin (200 mg/kg). On 22nd day of the experiment, animals were sacrificed, and their blood and brains were collected for neuro-biomarker analysis.
Diabetic-induced rats that received metformin, LEL and LEH exhibited considerably reduced levels of dopamine, serotonin, norepinephrine, NO, MDA, and AChE, BChE activities when compared to untreated diabetic animals. Additionally, rats with diabetes that received treatment with metformin, LEL and LEH displayed a noticeable increase in ENTPDase, Na/K ATPase, GST, CAT, GPx, and SOD activities when compared to the untreated diabetic rats. Histological examination revealed improved brain architecture in the treated groups in contrast to those in diabetic-induced rats.
The alkaloid-rich extracts of might be effective in normalizing brain damage caused by complications of diabetes mellitus.
研究表明，药用植物提取物有助于减少与糖尿病相关的神经元退化。在本研究中，评估了从[植物名称]叶片中提取的富含生物碱的提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠的神经保护作用。
通过已知程序进行富含生物碱的分析。将大鼠随机分为五个治疗组（n = 8）：正常对照组、糖尿病诱导大鼠（45mg/kg STZ），以及用低剂量[植物名称]叶片富含生物碱提取物（50mg/kg体重，LEL）和高剂量（100mg/kg体重，LEH）（300mg/kg和150mg/kg）治疗的糖尿病大鼠，还有二甲双胍（200mg/kg）治疗组。在实验的第22天，处死动物，收集其血液和大脑用于神经生物标志物分析。
与未治疗的糖尿病动物相比，接受二甲双胍、LEL和LEH治疗的糖尿病诱导大鼠的多巴胺、血清素、去甲肾上腺素、NO、MDA以及AChE、BChE活性水平显著降低。此外，与未治疗的糖尿病大鼠相比，接受二甲双胍、LEL和LEH治疗的糖尿病大鼠的ENTPDase、Na/K ATPase、GST、CAT、GPx和SOD活性显著增加。组织学检查显示，与糖尿病诱导大鼠相比，治疗组的脑结构有所改善。
[植物名称]富含生物碱的提取物可能对使糖尿病并发症引起的脑损伤正常化有效。
