Fusco da Costa Ana R, Fedrizzi Tarcisio, Lopes Maria L, Pecorari Monica, Oliveira da Costa Wana L, Giacobazzi Elisabetta, da Costa Bahia Jeann R, De Sanctis Veronica, Batista Lima Karla V, Bertorelli Roberto, Grottola Antonella, Fabio Anna, Mariottini Alessandro, Ferretti Pamela, Di Leva Francesca, Fregni Serpini Giulia, Tagliazucchi Sara, Rumpianesi Fabio, Jousson Olivier, Segata Nicola, Tortoli Enrico
Bacteriology and Mycology Section, Evandro Chagas Institute, Pará, Brazil.
NGS Facility, Laboratory of Biomolecular Sequence and Structure Analysis for Health, Centre for Integrative Biology, University of Trento, Italy.
Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):656-662. doi: 10.1099/ijs.0.068395-0. Epub 2014 Dec 8.
Fourteen mycobacterial strains isolated from pulmonary samples of independent patients in the state of Pará (Brazil), and three strains isolated in Italy, were characterized using a polyphasic approach. Thorough genetic investigation, including whole-genome sequencing, demonstrated that the strains belong to the M. simiae complex, being most closely related to Mycobacterium interjectum. For 14 of the strains, evidence emerged supporting their inclusion in a previously unreported species of the genus Mycobacterium, for which the name Mycobacterium paraense sp. nov. is proposed (type strain, IEC26(T) = DSM 46749(T) = CCUG 66121(T)). The novel species is characterized by slow growth, unpigmented or pale yellow scotochromogenic colonies, and a HPLC mycolic acid profile different from other known mycobacteria. In different genetic regions, high sequence microheterogeneity was detected.
从巴西帕拉州独立患者的肺部样本中分离出的14株分枝杆菌菌株，以及在意大利分离出的3株菌株，采用多相方法进行了鉴定。全面的基因研究，包括全基因组测序，表明这些菌株属于猿分枝杆菌复合群，与插入分枝杆菌关系最为密切。对于其中14株菌株，有证据支持将它们归入分枝杆菌属一个以前未报道的物种，为此提出了新种名巴西分枝杆菌（Mycobacterium paraense sp. nov.）（模式菌株，IEC26(T) = DSM 46749(T) = CCUG 66121(T)）。该新物种的特征为生长缓慢、无色素或浅黄色暗产色菌落，以及与其他已知分枝杆菌不同的高效液相色谱法分枝菌酸图谱。在不同的基因区域检测到了高度的序列微异质性。
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