Hu Qiang, Zhang Long, Hang Ping, Zhou Xi-Yi, Jia Wei-Bin, Li Shun-Peng, Jiang Jian-Dong
Department of Microbiology, Key Lab of Microbiology for Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095, Nanjing, PR China.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2622-2626. doi: 10.1099/ijsem.0.002887. Epub 2018 Jun 27.
A novel Gram-staining-negative, non-motile and rod-shaped bacterial strain ZQBW, which was isolated from a diuron-polluted soil collected near Nanjing, PR China, was investigated for its taxonomic position by a polyphasic approach. ZQBW grew well at pH 6.0-12.0 (optimum, pH 7.0), 26-35 °C (optimum, 30 °C) and up to 0.5 % NaCl (optimally the absence of NaCl) in R2A broth. The major fatty acids of ZQBW were C18 : 1ω7c (82.7 %) and C18 : 0 (5.3 %). The polar lipid profile included the major compounds phophatidylcholine, phosphatidylglycerol and phosphatidylmethylethanolamine. The only respiratory quinone was ubiquinone Q-10. The G+C content of genomic DNA was 67.0 mol%. Comparisons with 16S rRNA gene sequences revealed that ZQBW has the highest sequence similarities with members of the genus Tabrizicola (≤95.97 %), followed by Rhodobacter(≤95.96 %) and Falsirhodobacter(95.95 %) which all belong to the family Rhodobacteraceaein the phylum Proteobacteria. Photosynthesis genes pufLM were not found and photosynthesis pigments were not formed in ZQBW. On the basis of the results from chemotaxonomic, phenotypic and phylogenetic analysis, ZQBW represents a novel species of a novel genus, for which the name Xinfangfangia soli gen. nov., sp. nov. is proposed. The type strain is ZQBW (=KCTC 62102=CCTCC AB 2017177).
从中国南京附近受敌草隆污染的土壤中分离出一株新型革兰氏染色阴性、无运动性的杆状细菌菌株ZQBW，采用多相分类法对其分类地位进行了研究。ZQBW在R2A肉汤中，pH值为6.0 - 12.0（最适pH值7.0）、温度为26 - 35 °C（最适温度30 °C）以及NaCl浓度高达0.5 %（最适无NaCl）的条件下生长良好。ZQBW的主要脂肪酸为C18 : 1ω7c（82.7 %）和C18 : 0（5.3 %）。极性脂质谱包括主要化合物磷脂酰胆碱、磷脂酰甘油和磷脂酰甲基乙醇胺。唯一的呼吸醌为泛醌Q - 10。基因组DNA的G + C含量为67.0 mol%。与16S rRNA基因序列比较表明，ZQBW与塔布里齐菌属成员的序列相似性最高（≤95.97 %），其次是红杆菌属（≤95.96 %）和假红杆菌属（95.95 %），它们均属于变形菌门红杆菌科。在ZQBW中未发现光合作用基因pufLM，也未形成光合作用色素。基于化学分类、表型和系统发育分析结果，ZQBW代表一个新属的新物种，为此提出新属名为新芳方菌属（Xinfangfangia），新种名为土壤新芳方菌（Xinfangfangia soli）。模式菌株为ZQBW（=KCTC 62102 = CCTCC AB 2017177）。
